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Achieving long-term stability of lipid nanoparticles: examining the effect of pH, temperature, and lyophilization

Overview of attention for article published in International Journal of Nanomedicine, December 2016
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About this Attention Score

  • In the top 5% of all research outputs scored by Altmetric
  • One of the highest-scoring outputs from this source (#10 of 3,122)
  • High Attention Score compared to outputs of the same age (97th percentile)
  • High Attention Score compared to outputs of the same age and source (97th percentile)

Mentioned by

news
12 news outlets
twitter
12 tweeters

Citations

dimensions_citation
33 Dimensions

Readers on

mendeley
129 Mendeley
Title
Achieving long-term stability of lipid nanoparticles: examining the effect of pH, temperature, and lyophilization
Published in
International Journal of Nanomedicine, December 2016
DOI 10.2147/ijn.s123062
Pubmed ID
Authors

Rebecca Ball, Palak Bajaj, Kathryn Whitehead

Abstract

The broadest clinical application of siRNA therapeutics will be facilitated by drug-loaded delivery systems that maintain stability and potency for long times under ambient conditions. In the present study, we seek to better understand the stability and effect of storage conditions on lipidoid nanoparticles (LNPs), which have been previously shown by our group and others to potently deliver RNA to various cell and organ targets both in vitro and in vivo. Specifically, this study evaluates the influence of pH, temperature, and lyophilization on LNP efficacy in HeLa cells. When stored under aqueous conditions, we found that refrigeration (2°C) kept LNPs the most stable over 150 days compared to storage in the -20°C freezer or at room temperature. Because the pH of the storage buffer was not found to influence stability, it is suggested that the LNPs be stored under physiologically appropriate conditions (pH 7) for ease of use. Although aggregation and loss of efficacy were observed when LNPs were subjected to freeze-thaw cycles, their stability was retained with the use of the cryoprotectants, trehalose, and sucrose. Initially, lyophilization of the LNPs followed by reconstitution in aqueous buffer also led to reductions in efficacy, most likely due to aggregation upon reconstitution. Although the addition of ethanol to the reconstitution buffer restored efficacy, this approach is not ideal, as LNP solutions would require dialysis prior to use. Fortunately, we found that the addition of trehalose or sucrose to LNP solutions prior to lyophilization facilitated room temperature storage and reconstitution in aqueous buffer without diminishing delivery potency.

Twitter Demographics

The data shown below were collected from the profiles of 12 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 129 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 129 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 32 25%
Researcher 20 16%
Student > Bachelor 20 16%
Student > Master 17 13%
Other 10 8%
Other 12 9%
Unknown 18 14%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 25 19%
Biochemistry, Genetics and Molecular Biology 16 12%
Engineering 14 11%
Chemical Engineering 10 8%
Medicine and Dentistry 10 8%
Other 24 19%
Unknown 30 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 103. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 February 2021.
All research outputs
#228,762
of 17,060,012 outputs
Outputs from International Journal of Nanomedicine
#10
of 3,122 outputs
Outputs of similar age
#8,112
of 393,050 outputs
Outputs of similar age from International Journal of Nanomedicine
#2
of 81 outputs
Altmetric has tracked 17,060,012 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 98th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,122 research outputs from this source. They receive a mean Attention Score of 3.6. This one has done particularly well, scoring higher than 99% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 393,050 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 97% of its contemporaries.
We're also able to compare this research output to 81 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.