Title |
A sensitive and selective electrochemical biosensor for the determination of beta-amyloid oligomer by inhibiting the peptide-triggered in situ assembly of silver nanoparticles
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Published in |
International Journal of Nanomedicine, April 2017
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DOI | 10.2147/ijn.s132776 |
Pubmed ID | |
Authors |
Yun Xing, Xiao-Zhen Feng, Lipeng Zhang, Jiating Hou, Guo-Cheng Han, Zhencheng Chen |
Abstract |
Soluble beta-amyloid (Aβ) oligomer is believed to be the most important toxic species in the brain of Alzheimer's disease (AD) patients. Thus, it is critical to develop a simple method for the selective detection of Aβ oligomer with low cost and high sensitivity. In this paper, we report an electrochemical method for the detection of Aβ oligomer with a peptide as the bioreceptor and silver nanoparticle (AgNP) aggregates as the redox reporters. This strategy is based on the conversion of AgNP-based colorimetric assay into electrochemical analysis. Specifically, the peptide immobilized on the electrode surface and presented in solution triggered together the in situ formation of AgNP aggregates, which produced a well-defined electrochemical signal. However, the specific binding of Aβ oligomer to the immobilized peptide prevented the in situ assembly of AgNPs. As a result, a poor electrochemical signal was observed. The detection limit of the method was found to be 6 pM. Furthermore, the amenability of this method for the analysis of Aβ oligomer in serum and artificial cerebrospinal fluid (aCSF) samples was demonstrated. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 67 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 15% |
Student > Ph. D. Student | 8 | 12% |
Student > Bachelor | 8 | 12% |
Student > Doctoral Student | 4 | 6% |
Student > Master | 4 | 6% |
Other | 12 | 18% |
Unknown | 21 | 31% |
Readers by discipline | Count | As % |
---|---|---|
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Chemistry | 10 | 15% |
Engineering | 6 | 9% |
Agricultural and Biological Sciences | 4 | 6% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 4% |
Other | 6 | 9% |
Unknown | 27 | 40% |