Title |
Determination of the spatiotemporal dependence of Pseudomonas aeruginosa biofilm viability after treatment with NLC-colistin
|
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Published in |
International Journal of Nanomedicine, June 2017
|
DOI | 10.2147/ijn.s138763 |
Pubmed ID | |
Authors |
Eulalia Sans-Serramitjana, Marta Jorba, José Luis Pedraz, Teresa Vinuesa, Miguel Viñas |
Abstract |
The emergence of colistin-resistant Pseudomonas aeruginosa in cystic fibrosis (CF) patients, particularly after long-term inhalation treatments, has been recently reported. Nanoen-capsulation may enable preparations to overcome the limitations of conventional pharmaceutical forms. We have determined the time-dependent viability of P. aeruginosa biofilms treated with both free and nanoencapsulated colistin. We also examined the relationship between the optimal anti-biofilm activity of nanostructured lipid carrier (NLC)-colistin and the structural organization of the biofilm itself. The results showed the more rapid killing of P. aeruginosa bacterial biofilms by NLC-colistin than by free colistin. However, the two formulations did not differ in terms of the final percentages of living and dead cells, which were higher in the inner than in the outer layers of the treated biofilms. The effective anti-biofilm activity of NLC-colistin and its faster killing effect recommend further studies of its use over free colistin in the treatment of P. aeruginosa infections in CF patients. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
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Scientists | 1 | 50% |
Members of the public | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 43 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 6 | 14% |
Researcher | 5 | 12% |
Student > Master | 5 | 12% |
Student > Bachelor | 5 | 12% |
Unspecified | 3 | 7% |
Other | 12 | 28% |
Unknown | 7 | 16% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 9 | 21% |
Pharmacology, Toxicology and Pharmaceutical Science | 7 | 16% |
Agricultural and Biological Sciences | 5 | 12% |
Biochemistry, Genetics and Molecular Biology | 3 | 7% |
Immunology and Microbiology | 3 | 7% |
Other | 6 | 14% |
Unknown | 10 | 23% |