| Title |
Indole and synthetic derivative activate chaperone expression to reduce polyQ aggregation in SCA17 neuronal cell and slice culture models
|
|---|---|
| Published in |
Drug Design, Development and Therapy, October 2014
|
| DOI | 10.2147/dddt.s67376 |
| Pubmed ID | |
| Authors |
Pin-Jui Kung, Yu-Chen Tao, Ho-Chiang Hsu, Wan-Ling Chen, Te-Hsien Lin, Donala Janreddy, Ching-Fa Yao, Kuo-Hsuan Chang, Jung-Yaw Lin, Ming-Tsan Su, Chung-Hsin Wu, Guey-Jen Lee-Chen, Hsiu-Mei Hsieh-Li |
| Abstract |
In spinocerebellar ataxia type 17 (SCA17), the expansion of a translated CAG repeat in the TATA box binding protein (TBP) gene results in a long polyglutamine (polyQ) tract in the TBP protein, leading to intracellular accumulation of aggregated TBP and cell death. The molecular chaperones act in preventing protein aggregation to ameliorate downstream harmful events. In this study, we used Tet-On SH-SY5Y cells with inducible SCA17 TBP/Q79-green fluorescent protein (GFP) expression to test indole and synthetic derivative NC001-8 for neuroprotection. We found that indole and NC001-8 up-regulated chaperone expression to reduce polyQ aggregation in neuronal differentiated TBP/Q79 cells. The effects on promoting neurite outgrowth and on reduction of aggregation on Purkinje cells were also confirmed with cerebellar primary and slice cultures of SCA17 transgenic mice. Our results demonstrate how indole and derivative NC001-8 reduce polyQ aggregation to support their therapeutic potentials in SCA17 treatment. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 28 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 28 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 9 | 32% |
| Researcher | 4 | 14% |
| Professor | 3 | 11% |
| Student > Doctoral Student | 2 | 7% |
| Student > Bachelor | 1 | 4% |
| Other | 3 | 11% |
| Unknown | 6 | 21% |
| Readers by discipline | Count | As % |
|---|---|---|
| Neuroscience | 7 | 25% |
| Biochemistry, Genetics and Molecular Biology | 4 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 7% |
| Agricultural and Biological Sciences | 2 | 7% |
| Chemistry | 2 | 7% |
| Other | 5 | 18% |
| Unknown | 6 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 19 July 2016.
All research outputs
#8,277,187
of 25,411,814 outputs
Outputs from Drug Design, Development and Therapy
#581
of 2,271 outputs
Outputs of similar age
#84,273
of 265,585 outputs
Outputs of similar age from Drug Design, Development and Therapy
#18
of 49 outputs
Altmetric has tracked 25,411,814 research outputs across all sources so far. This one has received more attention than most of these and is in the 66th percentile.
So far Altmetric has tracked 2,271 research outputs from this source. They typically receive a little more attention than average, with a mean Attention Score of 7.1. This one has gotten more attention than average, scoring higher than 72% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 265,585 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 67% of its contemporaries.
We're also able to compare this research output to 49 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.