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Engineered exosome-mediated delivery of functionally active miR-26a and its enhanced suppression effect in HepG2 cells

Overview of attention for article published in International Journal of Nanomedicine, January 2018
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Title
Engineered exosome-mediated delivery of functionally active miR-26a and its enhanced suppression effect in HepG2 cells
Published in
International Journal of Nanomedicine, January 2018
DOI 10.2147/ijn.s154458
Pubmed ID
Authors

Gaofeng Liang, Shu Kan, Yanliang Zhu, Shuying Feng, Wenpo Feng, Shegan Gao

Abstract

Exosomes are closed-membrane nanovesicles that are secreted by a variety of cells and exist in most body fluids. Recent studies have demonstrated the potential of exosomes as natural vehicles that target delivery of functional small RNA and chemotherapeutics to diseased cells. In this study, we introduce a new approach for the targeted delivery of exosomes loaded with functional miR-26a to scavenger receptor class B type 1-expressing liver cancer cells. The tumor cell-targeting function of these engineered exosomes was introduced by expressing in 293T cell hosts, the gene fusion between the transmembrane protein of CD63 and a sequence from Apo-A1. The exosomes harvested from these 293T cells were loaded with miR-26a via electroporation. The engineered exosomes were shown to bind selectively to HepG2 cells via the scavenger receptor class B type 1-Apo-A1 complex and then internalized by receptor-mediated endocytosis. The release of miR-26a in exosome-treated HepG2 cells upregulated miR-26a expression and decreased the rates of cell migration and proliferation. We also presented evidence that suggest cell growth was inhibited by miR-26a-mediated decreases in the amounts of key proteins that regulate the cell cycle. Our gene delivery strategy can be adapted to treat a broad spectrum of cancers by expressing proteins on the surface of miRNA-loaded exosomes that recognize specific biomarkers on the tumor cell.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 65 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 65 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 11 17%
Student > Ph. D. Student 11 17%
Researcher 6 9%
Student > Bachelor 6 9%
Student > Doctoral Student 5 8%
Other 14 22%
Unknown 12 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 15 23%
Agricultural and Biological Sciences 13 20%
Medicine and Dentistry 5 8%
Unspecified 5 8%
Engineering 5 8%
Other 7 11%
Unknown 15 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 04 February 2018.
All research outputs
#11,078,276
of 12,459,998 outputs
Outputs from International Journal of Nanomedicine
#2,185
of 2,461 outputs
Outputs of similar age
#287,411
of 339,735 outputs
Outputs of similar age from International Journal of Nanomedicine
#90
of 110 outputs
Altmetric has tracked 12,459,998 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 2,461 research outputs from this source. They receive a mean Attention Score of 3.0. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 339,735 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 110 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.