↓ Skip to main content

Dove Medical Press

Article Metrics

Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells

Overview of attention for article published in International Journal of Nanomedicine, March 2015
Altmetric Badge

About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Good Attention Score compared to outputs of the same age (71st percentile)
  • High Attention Score compared to outputs of the same age and source (95th percentile)

Mentioned by

patent
5 patents

Citations

dimensions_citation
36 Dimensions

Readers on

mendeley
71 Mendeley
Title
Peptide-enhanced mRNA transfection in cultured mouse cardiac fibroblasts and direct reprogramming towards cardiomyocyte-like cells
Published in
International Journal of Nanomedicine, March 2015
DOI 10.2147/ijn.s75124
Pubmed ID
Authors

Niren Murthy, Kunwoo Lee, Pengzhi Yu, Nithya Lingampalli, Hyun Jin Kim, Richard Tang

Abstract

The treatment of myocardial infarction is a major challenge in medicine due to the inability of heart tissue to regenerate. Direct reprogramming of endogenous cardiac fibroblasts into functional cardiomyocytes via the delivery of transcription factor mRNAs has the potential to regenerate cardiac tissue and to treat heart failure. Even though mRNA delivery to cardiac fibroblasts has the therapeutic potential, mRNA transfection in cardiac fibroblasts has been challenging. Herein, we develop an efficient mRNA transfection in cultured mouse cardiac fibroblasts via a polyarginine-fused heart-targeting peptide and lipofectamine complex, termed C-Lipo and demonstrate the partial direct reprogramming of cardiac fibroblasts towards cardiomyocyte cells. C-Lipo enabled the mRNA-induced direct cardiac reprogramming due to its efficient transfection with low toxicity, which allowed for multiple transfections of Gata4, Mef2c, and Tbx5 (GMT) mRNAs for a period of 2 weeks. The induced cardiomyocyte-like cells had α-MHC promoter-driven GFP expression and striated cardiac muscle structure from α-actinin immunohistochemistry. GMT mRNA transfection of cultured mouse cardiac fibroblasts via C-Lipo significantly increased expression of the cardiomyocyte marker genes, Actc1, Actn2, Gja1, Hand2, and Tnnt2, after 2 weeks of transfection. Moreover, this study provides the first direct evidence that the stoichiometry of the GMT reprogramming factors influence the expression of cardiomyocyte marker genes. Our results demonstrate that mRNA delivery is a potential approach for cardiomyocyte generation.

Mendeley readers

The data shown below were compiled from readership statistics for 71 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 71 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 14 20%
Student > Ph. D. Student 13 18%
Student > Bachelor 10 14%
Researcher 9 13%
Student > Doctoral Student 6 8%
Other 6 8%
Unknown 13 18%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 22 31%
Agricultural and Biological Sciences 12 17%
Medicine and Dentistry 7 10%
Chemistry 4 6%
Engineering 3 4%
Other 7 10%
Unknown 16 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 23 March 2022.
All research outputs
#4,235,193
of 20,918,346 outputs
Outputs from International Journal of Nanomedicine
#341
of 3,520 outputs
Outputs of similar age
#83,051
of 297,915 outputs
Outputs of similar age from International Journal of Nanomedicine
#3
of 44 outputs
Altmetric has tracked 20,918,346 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 3,520 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 297,915 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 44 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 95% of its contemporaries.