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Dove Medical Press

Flow cytometry for intracellular SPION quantification: specificity and sensitivity in comparison with spectroscopic methods

Overview of attention for article published in International Journal of Nanomedicine, June 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (69th percentile)
  • High Attention Score compared to outputs of the same age and source (86th percentile)

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2 X users
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Citations

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60 Mendeley
Title
Flow cytometry for intracellular SPION quantification: specificity and sensitivity in comparison with spectroscopic methods
Published in
International Journal of Nanomedicine, June 2015
DOI 10.2147/ijn.s82714
Pubmed ID
Authors

Ralf P Friedrich, Christina Janko, Marina Poettler, Philipp Tripal, Jan Zaloga, Iwona Cicha, Stephan Dürr, Johannes Nowak, Stefan Odenbach, Ioana Slabu, Maik Liebl, Lutz Trahms, Marcus Stapf, Ingrid Hilger, Stefan Lyer, Christoph Alexiou

Abstract

Due to their special physicochemical properties, iron nanoparticles offer new promising possibilities for biomedical applications. For bench to bedside translation of super-paramagnetic iron oxide nanoparticles (SPIONs), safety issues have to be comprehensively clarified. To understand concentration-dependent nanoparticle-mediated toxicity, the exact quantification of intracellular SPIONs by reliable methods is of great importance. In the present study, we compared three different SPION quantification methods (ultraviolet spectrophotometry, magnetic particle spectroscopy, atomic adsorption spectroscopy) and discussed the shortcomings and advantages of each method. Moreover, we used those results to evaluate the possibility to use flow cytometric technique to determine the cellular SPION content. For this purpose, we correlated the side scatter data received from flow cytometry with the actual cellular SPION amount. We showed that flow cytometry provides a rapid and reliable method to assess the cellular SPION content. Our data also demonstrate that internalization of iron oxide nanoparticles in human umbilical vein endothelial cells is strongly dependent to the SPION type and results in a dose-dependent increase of toxicity. Thus, treatment with lauric acid-coated SPIONs (SEON(LA)) resulted in a significant increase in the intensity of side scatter and toxicity, whereas SEON(LA) with an additional protein corona formed by bovine serum albumin (SEON(LA-BSA)) and commercially available Rienso(®) particles showed only a minimal increase in both side scatter intensity and cellular toxicity. The increase in side scatter was in accordance with the measurements for SPION content by the atomic adsorption spectroscopy reference method. In summary, our data show that flow cytometry analysis can be used for estimation of uptake of SPIONs by mammalian cells and provides a fast tool for scientists to evaluate the safety of nanoparticle products.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 60 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Spain 1 2%
Unknown 59 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 20 33%
Student > Bachelor 8 13%
Student > Master 7 12%
Researcher 6 10%
Student > Doctoral Student 2 3%
Other 5 8%
Unknown 12 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 17 28%
Agricultural and Biological Sciences 7 12%
Chemistry 5 8%
Engineering 4 7%
Pharmacology, Toxicology and Pharmaceutical Science 3 5%
Other 7 12%
Unknown 17 28%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 January 2024.
All research outputs
#7,355,485
of 25,371,288 outputs
Outputs from International Journal of Nanomedicine
#814
of 4,123 outputs
Outputs of similar age
#81,661
of 281,399 outputs
Outputs of similar age from International Journal of Nanomedicine
#14
of 109 outputs
Altmetric has tracked 25,371,288 research outputs across all sources so far. This one has received more attention than most of these and is in the 69th percentile.
So far Altmetric has tracked 4,123 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done well, scoring higher than 78% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,399 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 86% of its contemporaries.