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Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions

Overview of attention for article published in Clinical Ophthalmology, October 2015
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (52nd percentile)
  • Average Attention Score compared to outputs of the same age and source

Mentioned by

twitter
1 tweeter
googleplus
1 Google+ user

Citations

dimensions_citation
3 Dimensions

Readers on

mendeley
17 Mendeley
Title
Acanthamoeba encystment: multifactorial effects of buffers, biocides, and demulcents present in contact lens care solutions
Published in
Clinical Ophthalmology, October 2015
DOI 10.2147/opth.s88199
Pubmed ID
Authors

Marjorie Rah, Christopher Kovacs, Shawn Lynch, Kimberly Millard, Timothy Morris

Abstract

To determine whether agents which are purportedly capable of inducing encystment of Acanthamoeba can recapitulate the signal when tested in differing formulations. In accordance with the International Standard ISO 19045, Acanthamoeba castellanii ATCC 50370 trophozoites were cultured in antibiotic-free axenic medium, treated with test solutions, and encystment rates plus viability were measured via bright field and fluorescent microscopy. Test solutions included phosphate-buffered saline (PBS), borate-buffered saline, biguanide- and hydrogen peroxide (H2O2)-based biocides, propylene glycol (PG) and povidone (POV) ophthalmic demulcents, and one-step H2O2-based contact lens disinfection systems. Only PBS solutions with 0.25 ppm polyaminopropyl biguanide (PAPB) and increasing concentrations of PG and POV stimulated A. castellanii encystment in a dose-dependent manner, whereas PBS solutions containing 3% H2O2 and increasing concentrations of PG and POV did not stimulate encystment. Borate-buffered saline and PBS/citrate solutions containing PG also did not stimulate encystment. In addition, no encystment was observed after 24 hours, 7 days, or 14 days of exposures of trophozoites to one-step H2O2 contact lens disinfection products or related solutions. The lack of any encystment observed when trophozoites were treated with existing or new one-step H2O2 contact lens care products, as well as when trophozoites were exposed to various related test solutions, confirms that Acanthamoeba encystment is a complex process which depends upon simultaneous contributions of multiple factors including buffers, biocides, and demulcents.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 6 35%
Student > Master 2 12%
Unspecified 2 12%
Researcher 2 12%
Student > Ph. D. Student 2 12%
Other 3 18%
Readers by discipline Count As %
Immunology and Microbiology 5 29%
Unspecified 3 18%
Biochemistry, Genetics and Molecular Biology 2 12%
Pharmacology, Toxicology and Pharmaceutical Science 2 12%
Medicine and Dentistry 2 12%
Other 3 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 October 2015.
All research outputs
#7,208,395
of 12,488,808 outputs
Outputs from Clinical Ophthalmology
#538
of 1,597 outputs
Outputs of similar age
#111,300
of 250,963 outputs
Outputs of similar age from Clinical Ophthalmology
#34
of 84 outputs
Altmetric has tracked 12,488,808 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,597 research outputs from this source. They receive a mean Attention Score of 3.2. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 250,963 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 52% of its contemporaries.
We're also able to compare this research output to 84 others from the same source and published within six weeks on either side of this one. This one is in the 47th percentile – i.e., 47% of its contemporaries scored the same or lower than it.