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IL-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line H446

Overview of attention for article published in OncoTargets and therapy, September 2018
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Title
IL-8 regulates the stemness properties of cancer stem cells in the small-cell lung cancer cell line H446
Published in
OncoTargets and therapy, September 2018
DOI 10.2147/ott.s161760
Pubmed ID
Authors

Fang Jin, Yajing Miao, Pengyu Xu, Xiaofei Qiu

Abstract

Cancer stem cells (CSCs) are a small population of cancer cells located within a tumor that are highly tumorigenic, capable of tumor initiation, and resistant to cancer therapies. We identified the potential genes involved in regulating stemness properties and investigated the mechanisms in small-cell lung cancer (SCLC). Whole transcriptome sequencing technology was used to screen the potential genes involved in regulating stemness properties from SCLC-SCs (uPAR+) and differentiated cells (uPAR-) in the H446 cell line. The selected genes were validated by quantitative reverse transcription PCR and ELISAs. The effect of IL-8 on stemness of sphere-forming cells was determined through tumor sphere formation, wound healing migration, and in vivo tumorigenesis assays. In our study, uPAR+ and uPAR- cells showed different gene expression profiles. IL-8 was upregulated in SCLC sphere-forming cells. Blocking IL-8 expression with siRNA led to loss of stemness, including the self-renewal capability, migration, expression of stemness-related genes, and in vivo tumorigenicity, in sphere-forming cells. Consistently, exogenously added IL-8 enhanced stemness properties in parental cells. IL-8 was upregulated in SCLC sphere-forming cells, and critical for the acquisition and/or maintenance of the stemness features in the SCLC cell line H446. Our results suggest that blocking IL-8 signaling may provide a novel therapeutic approach for targeting SCLC-SCs and improve treatment and outcomes in SCLC.

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Mendeley readers

Mendeley readers

The data shown below were compiled from readership statistics for 26 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 26 100%

Demographic breakdown

Readers by professional status Count As %
Student > Bachelor 9 35%
Student > Master 3 12%
Student > Ph. D. Student 3 12%
Researcher 2 8%
Other 1 4%
Other 1 4%
Unknown 7 27%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 42%
Pharmacology, Toxicology and Pharmaceutical Science 4 15%
Agricultural and Biological Sciences 2 8%
Medicine and Dentistry 1 4%
Neuroscience 1 4%
Other 0 0%
Unknown 7 27%
Attention Score in Context

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2018.
All research outputs
#22,867,974
of 25,498,750 outputs
Outputs from OncoTargets and therapy
#2,088
of 3,021 outputs
Outputs of similar age
#303,005
of 346,028 outputs
Outputs of similar age from OncoTargets and therapy
#103
of 147 outputs
Altmetric has tracked 25,498,750 research outputs across all sources so far. This one is in the 1st percentile – i.e., 1% of other outputs scored the same or lower than it.
So far Altmetric has tracked 3,021 research outputs from this source. They receive a mean Attention Score of 2.9. This one is in the 1st percentile – i.e., 1% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 346,028 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 147 others from the same source and published within six weeks on either side of this one. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.