Title |
Fullerene mediates proliferation and cardiomyogenic differentiation of adipose-derived stem cells via modulation of MAPK pathway and cardiac protein expression
|
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Published in |
International Journal of Nanomedicine, January 2016
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DOI | 10.2147/ijn.s95863 |
Pubmed ID | |
Authors |
Tong Hao, Jin Zhou, Shuanghong Lü, Boguang Yang, Yan Wang, Wancai Fang, Xiaoxia Jiang, Qiuxia Lin, Junjie Li, Changyong Wang |
Abstract |
Zero-dimensional fullerenes can modulate the biological behavior of a variety of cell lines. However, the effects and molecular mechanisms of proliferation and cardiomyogenic differentiation in brown adipose-derived stem cells (BADSCs) are still unclear. In this study, we report the initial biological effects of fullerene-C60 on BADSCs at different concentrations. Results suggest that fullerene-C60 has no cytotoxic effects on BADSCs even at a concentration of 100 μg/mL. Fullerene-C60 improves the MAPK expression level and stem cell survival, proliferation, and cardiomyogenesis. Further, we found that the fullerene-C60 modulates cardiomyogenic differentiation. Fullerene-C60 improves the expression of cardiomyocyte-specific proteins (cTnT and α-sarcomeric actinin). At elevated concentration, fullerene-C60 reduces the incidence of diminished spontaneous cardiac differentiation of BADSCs with time. At the genetic level, fullerene-C60 (5 μg/mL) also improves the expression of cTnT. In addition, fullerene-C60 promotes the formation of gap junction among cells. These findings have important implications for clinical application of fullerenes in the treatment of myocardial infarction. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 3 | 75% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 32 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 22% |
Student > Bachelor | 5 | 16% |
Student > Master | 4 | 13% |
Student > Postgraduate | 3 | 9% |
Student > Doctoral Student | 2 | 6% |
Other | 7 | 22% |
Unknown | 4 | 13% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 22% |
Medicine and Dentistry | 6 | 19% |
Chemistry | 4 | 13% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 6% |
Neuroscience | 2 | 6% |
Other | 5 | 16% |
Unknown | 6 | 19% |