Title |
Use of biotin targeted methotrexate–human serum albumin conjugated nanoparticles to enhance methotrexate antitumor efficacy
|
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Published in |
International Journal of Nanomedicine, September 2011
|
DOI | 10.2147/ijn.s23949 |
Pubmed ID | |
Authors |
Azade Taheri, Rassoul Dinarvand, Faranak Salman Nouri, Mohammad Reza Khorramizadeh, Atefeh Taheri Borougeni, Pooria Mansoori, Fatemeh Atyabi |
Abstract |
Biotin molecules could be used as suitable targeting moieties in targeted drug delivery systems against tumors. To develop a biotin targeted drug delivery system, we employed human serum albumin (HSA) as a carrier. Methotrexate (MTX) molecules were conjugated to HSA. MTX-HSA nanoparticles (MTX-HSA NPs) were prepared from these conjugates by cross-linking the HSA molecules. Biotin molecules were then conjugated on the surface of MTX-HSA NPs. The anticancer efficacy of biotin targeted MTX-HSA NPs was evaluated in mice bearing 4T1 breast carcinoma. A single dose of biotin targeted MTX-HSA NPs showed stronger in vivo antitumor activity than non-targeted MTX-HSA NPs and free MTX. By 7 days after treatment, average tumor volume in the biotin targeted MTX-HSA NPs-treated group decreased to 17.6% of the initial tumor volume when the number of attached biotin molecules on MTX-HSA-NPs was the highest. Average tumor volume in non-targeted MTX-HSA NPs-treated mice grew rapidly and reached 250.7% of the initial tumor volume. Biotin targeted MTX-HSA NPs increased the survival of tumor-bearing mice to 47.5 ± 0.71 days and increased their life span up to 216.7%. Mice treated with biotin targeted MTX-HSA NPs showed slight body weight loss (8%) 21 days after treatment, whereas non-targeted MTX-HSA NPs treatment at the same dose caused a body weight loss of 27.05% ± 3.1%. |
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Geographical breakdown
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Portugal | 2 | 3% |
Iran, Islamic Republic of | 1 | 2% |
Russia | 1 | 2% |
United States | 1 | 2% |
Unknown | 60 | 92% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Master | 14 | 22% |
Researcher | 7 | 11% |
Student > Ph. D. Student | 7 | 11% |
Student > Bachelor | 7 | 11% |
Student > Doctoral Student | 4 | 6% |
Other | 12 | 18% |
Unknown | 14 | 22% |
Readers by discipline | Count | As % |
---|---|---|
Pharmacology, Toxicology and Pharmaceutical Science | 11 | 17% |
Chemistry | 9 | 14% |
Biochemistry, Genetics and Molecular Biology | 6 | 9% |
Agricultural and Biological Sciences | 6 | 9% |
Materials Science | 5 | 8% |
Other | 12 | 18% |
Unknown | 16 | 25% |