Title |
Long-term persistence of IgE anti-influenza A HIN1 virus antibodies in serum of children and adults following influenza A vaccination with subsequent H1N1 infection: a case study
|
---|---|
Published in |
Journal of Inflammation Research, October 2012
|
DOI | 10.2147/jir.s34152 |
Pubmed ID | |
Authors |
Tamar A Smith-Norowitz, Melanie Kusonruksa, Darrin Wong, Moshe M Norowitz, Rauno Joks, Helen G Durkin, Martin H Bluth |
Abstract |
The role of immunoglobulin (Ig) E in immunity against influenza A H1N1 has not been studied. Total serum IgE and specific IgE and IgG anti-H1N1 virus responses were studied in children and adults (n = 2) who received influenza virus vaccination (Flumist(®) or Fluzone(®) ) in autumn 2008 and 2009, and then subsequently became infected with the H1N1 virus in spring 2009. Twelve months after infection, antibodies in their serum were compared with those in the serum of subjects who were either vaccinated but not infected (n = 4) or nonvaccinated and noninfected subjects (n = 2), using UniCAP total IgE fluoroenzyme immunoassay, sodium dodecyl sulfate polyacrylamide gel electrophoresis, and Western blotting. Band sizes for the influenza virus (58, 56, 40, 30, 25, and 17 kDa) and H1N1 viral proteins (58, 56, 25, and 17 kDa) were determined, using sodium dodecyl sulfate polyacrylamide gel electrophoresis and Coomassie brilliant blue. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 2 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 50% |
Practitioners (doctors, other healthcare professionals) | 1 | 50% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 31% |
Student > Ph. D. Student | 3 | 23% |
Other | 3 | 23% |
Student > Master | 2 | 15% |
Unknown | 1 | 8% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 4 | 31% |
Agricultural and Biological Sciences | 3 | 23% |
Immunology and Microbiology | 2 | 15% |
Biochemistry, Genetics and Molecular Biology | 1 | 8% |
Chemical Engineering | 1 | 8% |
Other | 0 | 0% |
Unknown | 2 | 15% |