↓ Skip to main content

Dove Medical Press

Article Metrics

Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells

Overview of attention for article published in Clinical Ophthalmology, July 2016
Altmetric Badge

About this Attention Score

  • Average Attention Score compared to outputs of the same age
  • Good Attention Score compared to outputs of the same age and source (69th percentile)

Mentioned by

twitter
1 tweeter
peer_reviews
1 peer review site

Citations

dimensions_citation
2 Dimensions

Readers on

mendeley
4 Mendeley
Title
Biocompatibility of the vital dye Acid Violet-17 on retinal pigment epithelial cells
Published in
Clinical Ophthalmology, July 2016
DOI 10.2147/opth.s105695
Pubmed ID
Authors

Ayseguel Tura, Aizhan Alt, Julia Lüke, Salvatore Grisanti, Christos Haritoglou, Carsten H. Meyer, Khaled Nassar, Matthias Lüke

Abstract

To examine the viability and differentiation of retinal pigment epithelial (RPE) cells after exposure to the vital dye Acid Violet-17 (AV-17). Bovine RPE cells were incubated with AV-17 (0.0625-0.5 mg/mL) for 30 seconds or 5 minutes. Viability was determined by live/dead staining, cleaved CASP3 immunostainings, and MTT test. Actin cytoskeleton was visualized by Alexa 488-phalloidin. Immunocytochemistry was performed to determine the levels of ZO-1, CTNNB1, and KRT19. Exposure to AV-17 at the concentrations of 0.25-0.5 mg/mL resulted in a dose-dependent decrease in viability, the loss of ZO-1 from tight junctions, translocation of CTNNB1 into the cytoplasm and nucleus, disarrangement of the actin cytoskeleton, and a slight increase in KRT19. AV-17 at a concentration <0.125 mg/mL is likely to be well tolerated by the RPE cells, whereas the concentrations from 0.25 mg/mL onward can reduce viability and induce dedifferentiation particularly after long-term exposure.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 4 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 4 100%

Demographic breakdown

Readers by professional status Count As %
Student > Doctoral Student 1 25%
Student > Bachelor 1 25%
Lecturer > Senior Lecturer 1 25%
Student > Master 1 25%
Readers by discipline Count As %
Medicine and Dentistry 2 50%
Computer Science 1 25%
Biochemistry, Genetics and Molecular Biology 1 25%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2017.
All research outputs
#7,208,400
of 12,488,808 outputs
Outputs from Clinical Ophthalmology
#537
of 1,597 outputs
Outputs of similar age
#123,082
of 263,212 outputs
Outputs of similar age from Clinical Ophthalmology
#18
of 76 outputs
Altmetric has tracked 12,488,808 research outputs across all sources so far. This one is in the 40th percentile – i.e., 40% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,597 research outputs from this source. They receive a mean Attention Score of 3.2. This one has gotten more attention than average, scoring higher than 59% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 263,212 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 50% of its contemporaries.
We're also able to compare this research output to 76 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 69% of its contemporaries.