↓ Skip to main content

Dove Medical Press

Article Metrics

Genetic and chemical knockdown: a complementary strategy for evaluating an anti-infective target

Overview of attention for article published in Advances and Applications in Bioinformatics and Chemistry : AABC, February 2013
Altmetric Badge

Mentioned by

facebook
1 Facebook page

Citations

dimensions_citation
9 Dimensions

Readers on

mendeley
23 Mendeley
Title
Genetic and chemical knockdown: a complementary strategy for evaluating an anti-infective target
Published in
Advances and Applications in Bioinformatics and Chemistry : AABC, February 2013
DOI 10.2147/aabc.s39198
Pubmed ID
Authors

Vasanthi Ramachandran, Vasanthi Ramachandran, Ragini Singh, Sylvia Yang, Tundugur Ragadeepthi, Subrat Mohapatra, Swati Khandelwal, Sanjana Patel

Abstract

The equity of a drug target is principally evaluated by its genetic vulnerability with tools ranging from antisense- and microRNA-driven knockdowns to induced expression of the target protein. In order to upgrade the process of antibacterial target identification and discern its most effective type of inhibition, an in silico toolbox that evaluates its genetic and chemical vulnerability leading either to stasis or cidal outcome was constructed and validated. By precise simulation and careful experimentation using enolpyruvyl shikimate-3-phosphate synthase and its specific inhibitor glyphosate, it was shown that genetic knockdown is distinct from chemical knockdown. It was also observed that depending on the particular mechanism of inhibition, viz competitive, uncompetitive, and noncompetitive, the antimicrobial potency of an inhibitor could be orders of magnitude different. Susceptibility of Escherichia coli to glyphosate and the lack of it in Mycobacterium tuberculosis could be predicted by the in silico platform. Finally, as predicted and simulated in the in silico platform, the translation of growth inhibition to a cidal effect was able to be demonstrated experimentally by altering the carbon source from sorbitol to glucose.

Mendeley readers

The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 23 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 6 26%
Student > Bachelor 5 22%
Other 4 17%
Professor 2 9%
Unspecified 1 4%
Other 4 17%
Unknown 1 4%
Readers by discipline Count As %
Agricultural and Biological Sciences 13 57%
Biochemistry, Genetics and Molecular Biology 3 13%
Medicine and Dentistry 2 9%
Unspecified 1 4%
Immunology and Microbiology 1 4%
Other 1 4%
Unknown 2 9%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 08 February 2013.
All research outputs
#2,901,494
of 3,636,264 outputs
Outputs from Advances and Applications in Bioinformatics and Chemistry : AABC
#14
of 15 outputs
Outputs of similar age
#224,723
of 281,609 outputs
Outputs of similar age from Advances and Applications in Bioinformatics and Chemistry : AABC
#2
of 2 outputs
Altmetric has tracked 3,636,264 research outputs across all sources so far. This one is in the 2nd percentile – i.e., 2% of other outputs scored the same or lower than it.
So far Altmetric has tracked 15 research outputs from this source. They receive a mean Attention Score of 2.8. This one scored the same or higher as 1 of them.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 281,609 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 1st percentile – i.e., 1% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 2 others from the same source and published within six weeks on either side of this one.