| Title |
Amorphous silica nanoparticles trigger vascular endothelial cell injury through apoptosis and autophagy via reactive oxygen species-mediated MAPK/Bcl-2 and PI3K/Akt/mTOR signaling
|
|---|---|
| Published in |
International Journal of Nanomedicine, October 2016
|
| DOI | 10.2147/ijn.s112030 |
| Pubmed ID | |
| Authors |
Caixia Guo, Man Yang, Li Jing, Ji Wang, Yang Yu, Yang Li, Junchao Duan, Xianqing Zhou, Yanbo Li, Zhiwei Sun |
| Abstract |
Environmental exposure to silica nanoparticles (SiNPs) is inevitable due to their widespread application in industrial, commercial, and biomedical fields. In recent years, most investigators focus on the evaluation of cardiovascular effects of SiNPs in vivo and in vitro. Endothelial injury and dysfunction is now hypothesized to be a dominant mechanism in the development of cardiovascular diseases. This study aimed to explore interaction of SiNPs with endothelial cells, and extensively investigate the exact effects of reactive oxygen species (ROS) on the signaling molecules and cytotoxicity involved in SiNPs-induced endothelial injury. Significant induction of cytotoxicity as well as oxidative stress, apoptosis, and autophagy was observed in human umbilical vein endothelial cells following the SiNPs exposure (P<0.05). The oxidative stress was induced by ROS generation, leading to redox imbalance and lipid peroxidation. SiNPs induced mitochondrial dysfunction, characterized by membrane potential collapse, and elevated Bax and declined bcl-2 expression, ultimately leading to apoptosis, and also increased number of autophagosomes and autophagy marker proteins, such as LC3 and p62. Phosphorylated ERK, PI3K, Akt, and mTOR were significantly decreased, but phosphorylated JNK and p38 MAPK were increased in SiNPs-exposed endothelial cells. In contrast, all of these stimulation phenomena were effectively inhibited by N-acetylcysteine. The N-acetylcysteine supplement attenuated SiNPs-induced endothelial toxicity through inhibition of apoptosis and autophagy via MAPK/Bcl-2 and PI3K/Akt/mTOR signaling, as well as suppression of intracellular ROS property via activating antioxidant enzyme and Nrf2 signaling. In summary, the results demonstrated that SiNPs triggered autophagy and apoptosis via ROS-mediated MAPK/Bcl-2 and PI3K/Akt/mTOR signaling in endothelial cells, and subsequently disturbed the endothelial homeostasis and impaired endothelium. Our findings may provide experimental evidence and explanation for cardiovascular diseases triggered by SiNPs. Furthermore, results hint that the application of antioxidant may provide a novel way for safer use of nanomaterials. |
X Demographics
The data shown below were collected from the profiles of 37 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 10 | 27% |
| United Kingdom | 2 | 5% |
| Brazil | 1 | 3% |
| Puerto Rico | 1 | 3% |
| Uruguay | 1 | 3% |
| Australia | 1 | 3% |
| Germany | 1 | 3% |
| Canada | 1 | 3% |
| Ireland | 1 | 3% |
| Other | 0 | 0% |
| Unknown | 18 | 49% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 31 | 84% |
| Scientists | 3 | 8% |
| Practitioners (doctors, other healthcare professionals) | 3 | 8% |
Mendeley readers
The data shown below were compiled from readership statistics for 63 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 63 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 16% |
| Student > Bachelor | 7 | 11% |
| Student > Doctoral Student | 6 | 10% |
| Lecturer | 6 | 10% |
| Researcher | 6 | 10% |
| Other | 14 | 22% |
| Unknown | 14 | 22% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 17% |
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 13% |
| Medicine and Dentistry | 7 | 11% |
| Agricultural and Biological Sciences | 6 | 10% |
| Materials Science | 2 | 3% |
| Other | 6 | 10% |
| Unknown | 23 | 37% |
Attention Score in Context
This research output has an Altmetric Attention Score of 22. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 20 March 2022.
All research outputs
#1,755,362
of 25,757,133 outputs
Outputs from International Journal of Nanomedicine
#64
of 4,150 outputs
Outputs of similar age
#30,425
of 333,803 outputs
Outputs of similar age from International Journal of Nanomedicine
#3
of 109 outputs
Altmetric has tracked 25,757,133 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 93rd percentile: it's in the top 10% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,150 research outputs from this source. They receive a mean Attention Score of 4.7. This one has done particularly well, scoring higher than 98% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 333,803 tracked outputs that were published within six weeks on either side of this one in any source. This one has done particularly well, scoring higher than 90% of its contemporaries.
We're also able to compare this research output to 109 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.