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Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome

Overview of attention for article published in International Journal of Nanomedicine, October 2016
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Mentioned by

twitter
3 tweeters

Readers on

mendeley
11 Mendeley
Title
Efficient delivery of Notch1 siRNA to SKOV3 cells by cationic cholesterol derivative-based liposome
Published in
International Journal of Nanomedicine, October 2016
DOI 10.2147/ijn.s115367
Pubmed ID
Authors

Yunchun Zhao, Li Zhang, Shi-Sen Feng, Lu Hong, Hai-Li Zheng, Li-Li Chen, Xiao-Ling Zheng, Yi-Qing Ye, Meng-Dan Zhao, Wen-Xi Wang, Cai-Hong Zheng

Abstract

A novel cationic cholesterol derivative-based small interfering RNA (siRNA) interference strategy was suggested to inhibit Notch1 activation in SKOV3 cells for the gene therapy of ovarian cancer. The cationic cholesterol derivative, N-(cholesterylhemisuccinoyl-amino-3-propyl)-N, N-dimethylamine (DMAPA-chems) liposome, was incubated with siRNA at different nitrogen-to-phosphate ratios to form stabilized, near-spherical siRNA/DMAPA-chems nanoparticles with sizes of 100-200 nm and zeta potentials of 40-50 mV. The siRNA/DMAPA-chems nanoparticles protected siRNA from nuclease degradation in 25% fetal bovine serum. The nanoparticles exhibited high cell uptake and Notch1 gene knockdown efficiency in SKOV3 cells at an nitrogen-to-phosphate ratio of 100 and an siRNA concentration of 50 nM. They also inhibited the growth and promoted the apoptosis of SKOV3 cells. These results may provide the potential for using cationic cholesterol derivatives as efficient nonviral siRNA carriers for the suppression of Notch1 activation in ovarian cancer cells.

Twitter Demographics

The data shown below were collected from the profiles of 3 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 11 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 11 100%

Demographic breakdown

Readers by professional status Count As %
Student > Master 4 36%
Student > Ph. D. Student 3 27%
Student > Doctoral Student 1 9%
Researcher 1 9%
Student > Postgraduate 1 9%
Other 0 0%
Unknown 1 9%
Readers by discipline Count As %
Pharmacology, Toxicology and Pharmaceutical Science 3 27%
Biochemistry, Genetics and Molecular Biology 2 18%
Engineering 2 18%
Medicine and Dentistry 1 9%
Agricultural and Biological Sciences 1 9%
Other 0 0%
Unknown 2 18%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 21 October 2016.
All research outputs
#4,324,979
of 8,547,393 outputs
Outputs from International Journal of Nanomedicine
#707
of 1,874 outputs
Outputs of similar age
#127,351
of 249,233 outputs
Outputs of similar age from International Journal of Nanomedicine
#37
of 91 outputs
Altmetric has tracked 8,547,393 research outputs across all sources so far. This one is in the 46th percentile – i.e., 46% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,874 research outputs from this source. They receive a mean Attention Score of 2.5. This one is in the 45th percentile – i.e., 45% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 249,233 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 45th percentile – i.e., 45% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 91 others from the same source and published within six weeks on either side of this one. This one is in the 39th percentile – i.e., 39% of its contemporaries scored the same or lower than it.