Title |
Improved proliferation of antigen-specific cytolytic T lymphocytes using a multimodal nanovaccine
|
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Published in |
International Journal of Nanomedicine, November 2016
|
DOI | 10.2147/ijn.s112432 |
Pubmed ID | |
Authors |
Bo Li, Michael Siuta, Vanessa Bright, Dmitry Koktysh, Brittany K Matlock, Megan E Dumas, Meiying Zhu, Alex Holt, Donald Stec, Shenglou Deng, Paul B Savage, Sebastian Joyce, Wellington Pham |
Abstract |
The present study investigated the immunoenhancing property of our newly designed nanovaccine, that is, its ability to induce antigen-specific immunity. This study also evaluated the synergistic effect of a novel compound PBS-44, an α-galactosylceramide analog, in boosting the immune response induced by our nanovaccine. The nanovaccine was prepared by encapsulating ovalbumin (ova) and an adjuvant within the poly(lactic-co-glycolic acid) nanoparticles. Quantitative analysis of our study data showed that the encapsulated vaccine was physically and biologically stable; the core content of our nanovaccine was found to be released steadily and slowly, and nearly 90% of the core content was slowly released over the course of 25 days. The in vivo immunization studies exhibited that the nanovaccine induced stronger and longer immune responses compared to its soluble counterpart. Similarly, intranasal inhalation of the nanovaccine induced more robust antigen-specific CD8(+) T cell response than intraperitoneal injection of nanovaccine. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 2 | 40% |
Canada | 1 | 20% |
Unknown | 2 | 40% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 2 | 40% |
Members of the public | 2 | 40% |
Scientists | 1 | 20% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Germany | 1 | 4% |
Unknown | 23 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 4 | 17% |
Student > Bachelor | 3 | 13% |
Researcher | 3 | 13% |
Student > Ph. D. Student | 3 | 13% |
Professor | 2 | 8% |
Other | 4 | 17% |
Unknown | 5 | 21% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 4 | 17% |
Immunology and Microbiology | 4 | 17% |
Chemical Engineering | 2 | 8% |
Chemistry | 2 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 4% |
Other | 4 | 17% |
Unknown | 7 | 29% |