Title |
Curcumin sensitizes human gastric cancer cells to 5-fluorouracil through inhibition of the NFκB survival-signaling pathway
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Published in |
OncoTargets and therapy, December 2016
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DOI | 10.2147/ott.s118272 |
Pubmed ID | |
Authors |
Yanting Kang, Wanle Hu, Encheng Bai, Hailun Zheng, Zhiguo Liu, Jianzhang Wu, Rong Jin, Chengguang Zhao, Guang Liang |
Abstract |
Fluorouracil (5-FU) is the most commonly used chemotherapeutic agent for gastric cancer (GC). However, the occurrence of resistance to 5-FU treatment poses a major problem for its clinical efficacy. In this study, we found that the NFκB-signaling pathway can mediate 5-FU resistance in GC cells. We developed a 5-FU-resistant GC cell line named SGCR/5-FU and found that the 5-FU-induced resistance increased cytosolic IκBα degradation and promoted NFκB nuclear translocation in GC cells. These findings were further confirmed by the activation of the NFκB survival-signaling pathway in clinical specimens. Curcumin, a natural compound, can reverse 5-FU resistance and inhibits proliferation in GC cells by downregulating the NFκB-signaling pathway. Moreover, it can also decrease the expression level of TNFα messenger RNA. Flow cytometry and Western blot analysis results showed that the combination of curcumin and 5-FU caused synergistic inhibition of growth and induction of potent apoptosis in the resistant cancer cell lines in vitro. In conclusion, our results demonstrate that the combination of 5-FU and curcumin could be further developed as a potential therapy for human GC. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United States | 1 | 33% |
Unknown | 2 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 3 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 29 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 21% |
Researcher | 4 | 14% |
Student > Bachelor | 2 | 7% |
Other | 2 | 7% |
Student > Master | 2 | 7% |
Other | 3 | 10% |
Unknown | 10 | 34% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 6 | 21% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 14% |
Medicine and Dentistry | 3 | 10% |
Arts and Humanities | 1 | 3% |
Agricultural and Biological Sciences | 1 | 3% |
Other | 1 | 3% |
Unknown | 13 | 45% |