Title |
Coating with spermine-pullulan polymer enhances adenoviral transduction of mesenchymal stem cells
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Published in |
International Journal of Nanomedicine, December 2016
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DOI | 10.2147/ijn.s109897 |
Pubmed ID | |
Authors |
Li Wan, Xinglei Yao, Francesco Faiola, Bojun Liu, Tianyuan Zhang, Yasuhiko Tabata, Hiroyuki Mizuguchi, Shinsaku Nakagawa, Jian-Qing Gao, Robert Chunhua Zhao |
Abstract |
Mesenchymal stem cells (MSCs) are adult stem cells with multilineage potential, which makes them attractive tools for regenerative medicine applications. Efficient gene transfer into MSCs is essential not only for basic research in developmental biology but also for therapeutic applications involving gene-modification in regenerative medicine. Adenovirus vectors (Advs) can efficiently and transiently introduce an exogenous gene into many cell types via their primary receptors, the coxsackievirus and adenovirus receptors, but not into MSCs, which are deficient in coxsackievirus and adenovirus receptors expression. To overcome this problem, we developed an Adv coated with a spermine-pullulan (SP) cationic polymer and investigated its physicochemical properties and internalization mechanisms. We demonstrated that the SP coating could enhance adenoviral transduction of MSCs without detectable cytotoxicity or effects on differentiation. Our results argue in favor of the potentiality of the SP-coated Adv as a prototype vector for efficient and safe transduction of MSCs. |
X Demographics
Geographical breakdown
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United States | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Professor | 3 | 19% |
Student > Master | 3 | 19% |
Researcher | 2 | 13% |
Lecturer | 1 | 6% |
Student > Ph. D. Student | 1 | 6% |
Other | 3 | 19% |
Unknown | 3 | 19% |
Readers by discipline | Count | As % |
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Biochemistry, Genetics and Molecular Biology | 4 | 25% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 19% |
Medicine and Dentistry | 2 | 13% |
Environmental Science | 1 | 6% |
Engineering | 1 | 6% |
Other | 0 | 0% |
Unknown | 5 | 31% |