| Title |
A peptide-mediated targeting gene delivery system for malignant glioma cells
|
|---|---|
| Published in |
International Journal of Nanomedicine, September 2013
|
| DOI | 10.2147/ijn.s44990 |
| Pubmed ID | |
| Authors |
Chuanwei Wang, Liping Ning, Hongwei Wang, Zaijun Lu, Xingang Li, Xiaoyong Fan, Xuping Wang, Yuguang Liu |
| Abstract |
Glioblastoma multiforme (GBM) is the most common and malignant glioma. Although there has been considerable progress in treatment strategies, the prognosis of many patients with GBM remains poor. In this work, polyethylenimine (PEI) and the VTWTPQAWFQWV (VTW) peptide were modified and synthesized into GBM-targeting nanoparticles. The transfection efficiency of U-87 (human glioblastoma) cells was evaluated using fluorescence microscopy and flow cytometry. Cell internalization was investigated to verify the nanoparticle delivery into the cytoplasm. Results showed that the methods of polymer conjugation and the amount of VTW peptide were important factors to polymer synthesis and transfection. The PEI-VTW20 nanoparticles increased the transfection efficiency significantly. This report describes the use of VTW peptide-based PEI nanoparticles for intracellular gene delivery in a GBM cell-specific manner. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 23 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 23 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 7 | 30% |
| Student > Master | 4 | 17% |
| Student > Bachelor | 4 | 17% |
| Other | 1 | 4% |
| Student > Doctoral Student | 1 | 4% |
| Other | 2 | 9% |
| Unknown | 4 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 26% |
| Medicine and Dentistry | 5 | 22% |
| Biochemistry, Genetics and Molecular Biology | 3 | 13% |
| Agricultural and Biological Sciences | 2 | 9% |
| Chemical Engineering | 1 | 4% |
| Other | 3 | 13% |
| Unknown | 3 | 13% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 24 September 2013.
All research outputs
#16,721,717
of 25,374,647 outputs
Outputs from International Journal of Nanomedicine
#2,087
of 4,123 outputs
Outputs of similar age
#129,587
of 212,478 outputs
Outputs of similar age from International Journal of Nanomedicine
#64
of 104 outputs
Altmetric has tracked 25,374,647 research outputs across all sources so far. This one is in the 32nd percentile – i.e., 32% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,123 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
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We're also able to compare this research output to 104 others from the same source and published within six weeks on either side of this one. This one is in the 22nd percentile – i.e., 22% of its contemporaries scored the same or lower than it.