| Title |
Tumor-specific hyperthermia with aptamer-tagged superparamagnetic nanoparticles
|
|---|---|
| Published in |
International Journal of Nanomedicine, December 2013
|
| DOI | 10.2147/ijn.s52539 |
| Pubmed ID | |
| Authors |
Katarzyna Pala, Anna Serwotka, Filip Jeleń, Piotr Jakimowicz, Jacek Otlewski |
| Abstract |
Targeted therapy is a method owing to its limited side effect profile, particularly in cancer treatment. Magnetic hyperthermia, which is induced by nanoparticles (NPs) conjugated with targeting agents, can be useful in combination with chemo- or radiotherapy. In this paper, we constructed dextran-coated ferric oxide NPs conjugated with specific anti-human epidermal growth factor receptor (HER2) aptamer and used them to induce magnetic hyperthermia in cultured cells. The specificity of the tagged NPs was determined by studying their effect relative to that of non-tagged NPs against two cell lines: human adenocarcinoma SK-BR3, overexpressing the HER2 receptor; and U-87 MG, a human glioblastoma epithelial cell line, not expressing HER2. In order to confirm the interaction of the tagged NPs with the cells we used, fluorescence microscopy and fluorescence-activated cell sorting analysis were performed. All of these experiments showed that the aptamer-tagged NPs were highly specific toward the HER2-expressing cells. In addition, a ninetyfold lower dose of the tagged NPs relative to that of the non-tagged NPs was needed to achieve ~50% cell killing by hyperthermia of the SK-BR3 cell line, while for the U-87 MG cells the viability level was close to 100%. These results show that targeted NPs can be applied at substantially lower doses than non-targeted ones to achieve similar effects of hyperthermia, which should greatly limit the side effects of treatment. |
X Demographics
The data shown below were collected from the profiles of 3 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 3 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 3 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 92 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United States | 1 | 1% |
| Poland | 1 | 1% |
| China | 1 | 1% |
| Unknown | 89 | 97% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 20 | 22% |
| Student > Ph. D. Student | 18 | 20% |
| Student > Master | 17 | 18% |
| Student > Bachelor | 7 | 8% |
| Other | 5 | 5% |
| Other | 9 | 10% |
| Unknown | 16 | 17% |
| Readers by discipline | Count | As % |
|---|---|---|
| Agricultural and Biological Sciences | 24 | 26% |
| Biochemistry, Genetics and Molecular Biology | 12 | 13% |
| Medicine and Dentistry | 8 | 9% |
| Chemistry | 7 | 8% |
| Physics and Astronomy | 4 | 4% |
| Other | 16 | 17% |
| Unknown | 21 | 23% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 18 December 2013.
All research outputs
#16,721,208
of 25,371,288 outputs
Outputs from International Journal of Nanomedicine
#2,087
of 4,123 outputs
Outputs of similar age
#198,816
of 320,954 outputs
Outputs of similar age from International Journal of Nanomedicine
#52
of 101 outputs
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So far Altmetric has tracked 4,123 research outputs from this source. They receive a mean Attention Score of 4.7. This one is in the 40th percentile – i.e., 40% of its peers scored the same or lower than it.
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