Title |
The long-term fate of mesenchymal stem cells labeled with magnetic resonance imaging-visible polymersomes in cerebral ischemia
|
---|---|
Published in |
International Journal of Nanomedicine, September 2017
|
DOI | 10.2147/ijn.s146742 |
Pubmed ID | |
Authors |
Xiaohui Duan, Liejing Lu, Yong Wang, Fang Zhang, Jiaji Mao, Minghui Cao, Bingling Lin, Xiang Zhang, Xintao Shuai, Jun Shen |
Abstract |
Understanding the long-term fate and potential mechanisms of mesenchymal stem cells (MSCs) after transplantation is essential for improving functional benefits of stem cell-based stroke treatment. Magnetic resonance imaging (MRI) is considered an attractive and clinically translatable tool for longitudinal tracking of stem cells, but certain controversies have arisen in this regard. In this study, we used SPION-loaded cationic polymersomes to label green fluorescent protein (GFP)-expressing MSCs to determine whether MRI can accurately reflect survival, long-term fate, and potential mechanisms of MSCs in ischemic stroke therapy. Our results showed that MSCs could improve the functional outcome and reduce the infarct volume of stroke in the brain. In vivo MRI can verify the biodistribution and migration of grafted cells when pre-labeled with SPION-loaded polymersome. The dynamic change of low signal volume on MRI can reflect the tendency of cell survival and apoptosis, but may overestimate long-term survival owing to the presence of iron-laden macrophages around cell graft. Only a small fraction of grafted cells survived up to 8 weeks after transplantation. A minority of these surviving cells were differentiated into astrocytes, but not into neurons. MSCs might exert their therapeutic effect via secreting paracrine factors rather than directing cell replacement through differentiation into neuronal and/or glial phenotypes. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 3 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 2 | 67% |
Practitioners (doctors, other healthcare professionals) | 1 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 46 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 8 | 17% |
Student > Bachelor | 8 | 17% |
Student > Master | 6 | 13% |
Researcher | 5 | 11% |
Student > Doctoral Student | 2 | 4% |
Other | 4 | 9% |
Unknown | 13 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 11 | 24% |
Neuroscience | 5 | 11% |
Biochemistry, Genetics and Molecular Biology | 4 | 9% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 7% |
Agricultural and Biological Sciences | 3 | 7% |
Other | 7 | 15% |
Unknown | 13 | 28% |