| Title |
Efficacy of neutral and negatively charged liposome-loaded gentamicin on planktonic bacteria and biofilm communities
|
|---|---|
| Published in |
International Journal of Nanomedicine, September 2017
|
| DOI | 10.2147/ijn.s141709 |
| Pubmed ID | |
| Authors |
Moayad Alhariri, Majed A Majrashi, Ali H Bahkali, Faisal S Almajed, Ali O Azghani, Mohammad A Khiyami, Essam J Alyamani, Sameera M Aljohani, Majed A Halwani |
| Abstract |
We investigated the efficacy of liposomal gentamicin formulations of different surface charges against Pseudomonas aeruginosa and Klebsiella oxytoca. The liposomal gentamicin formulations were prepared by the dehydration-rehydration method, and their sizes and zeta potential were measured. Gentamicin encapsulation efficiency inside the liposomal formulations was determined by microbiologic assay, and stability of the formulations in biologic fluid was evaluated for a period of 48 h. The minimum inhibitory concentration and the minimum bactericidal concentration were determined, and the in vitro time kill studies of the free form of gentamicin and liposomal gentamicin formulations were performed. The activities of liposomal gentamicin in preventing and reducing biofilm-forming P. aeruginosa and K. oxytoca were compared to those of free antibiotic. The sizes of the liposomal formulations ranged from 625 to 806.6 nm in diameter, with the zeta potential ranging from -0.22 to -31.7 mV. Gentamicin encapsulation efficiency inside the liposomal formulation ranged from 1.8% to 43.6%. The liposomes retained >60% of their gentamicin content during the 48 h time period. The minimum inhibitory concentration of neutral formulation was lower than that of free gentamicin (0.25 versus 1 mg/L for P. aeruginosa and 0.5 versus 1 mg/L for K. oxytoca). The negatively charged formulation exhibited the same bacteriostatic concentration as that of free gentamicin. The minimum bactericidal concentration of neutral liposomes on planktonic bacterial culture was twofold lower than that of free gentamicin, whereas the negatively charged formulations were comparable to free gentamicin. The killing time curve values for the neutral negatively charged formulation against planktonic P. aeruginosa and K. oxytoca were better than those of free gentamicin. Furthermore, liposomal formulations prevent the biofilm-formation ability of these strains better than free gentamicin. In summary, liposomal formulations could be an effective lipid nanoparticle to combat acute infections where planktonic bacteria are predominant. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 50% |
| Scientists | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 78 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 78 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 15 | 19% |
| Student > Master | 9 | 12% |
| Researcher | 9 | 12% |
| Student > Bachelor | 6 | 8% |
| Other | 3 | 4% |
| Other | 8 | 10% |
| Unknown | 28 | 36% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 8 | 10% |
| Biochemistry, Genetics and Molecular Biology | 7 | 9% |
| Pharmacology, Toxicology and Pharmaceutical Science | 7 | 9% |
| Medicine and Dentistry | 4 | 5% |
| Agricultural and Biological Sciences | 3 | 4% |
| Other | 19 | 24% |
| Unknown | 30 | 38% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 29 October 2017.
All research outputs
#17,292,294
of 25,382,440 outputs
Outputs from International Journal of Nanomedicine
#2,470
of 4,122 outputs
Outputs of similar age
#207,824
of 324,453 outputs
Outputs of similar age from International Journal of Nanomedicine
#53
of 102 outputs
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