Title |
Specific IgA against Pseudomonas aeruginosa in severe COPD
|
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Published in |
International Journal of Chronic Obstructive Pulmonary Disease, September 2017
|
DOI | 10.2147/copd.s141701 |
Pubmed ID | |
Authors |
Laura Millares, Sara Martí, Carmen Ardanuy, Josefina Liñares, Salud Santos, Jordi Dorca, Marian García-Nuñez, Sara Quero, Eduard Monsó |
Abstract |
The bronchial mucosa is protected by a specialized immune system focused on the prevention of colonization and infection by potentially pathogenic microorganisms (PPMs). Immunoglobulin A (IgA) is the principal antibody involved in this mechanism. A defective immune barrier may facilitate the recurrent presence of PPMs in COPD. The aim of this study was to determine IgA-mediated bronchial specific immune responses against Pseudomonas aeruginosa in stable patients with severe disease. COPD patients with good-quality sputum samples obtained during stability were included and classified according to the presence or absence of chronic bronchial colonization by P. aeruginosa. Levels of specific IgA for P. aeruginosa in sputum were determined by ELISA and expressed as ratios, using the pooled level of 10 healthy subjects as reference (optical density450 patient/control). Thirty-six stable COPD patients were included, 15 of whom had chronic colonization by P. aeruginosa. Levels of specific IgA against P. aeruginosa in stable non-colonized patients were lower than those in healthy subjects (IgA ratio: median =0.15 [interquartile range {IQR} 0.05-0.36]). Colonized patients had higher levels, (1.56 [IQR 0.59-2.79]) (p<0.001, Mann-Whitney U test), with figures equivalent but not exceeding the reference value. IgA-based immune response against P. aeruginosa was low in severe COPD patients. Levels of specific IgA against this microorganism were higher in colonized patients, but did not attain clear-cut levels above the reference. An impaired local response against P. aeruginosa may favor chronic colonization and recurrent infections in severe COPD. |
X Demographics
Geographical breakdown
Country | Count | As % |
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United Kingdom | 1 | 50% |
Unknown | 1 | 50% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 2 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 20 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 6 | 30% |
Student > Bachelor | 2 | 10% |
Lecturer > Senior Lecturer | 1 | 5% |
Librarian | 1 | 5% |
Other | 1 | 5% |
Other | 2 | 10% |
Unknown | 7 | 35% |
Readers by discipline | Count | As % |
---|---|---|
Immunology and Microbiology | 6 | 30% |
Medicine and Dentistry | 4 | 20% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Veterinary Science and Veterinary Medicine | 1 | 5% |
Unknown | 8 | 40% |