Title |
Quantum dots-based lateral flow immunoassay combined with image analysis for semiquantitative detection of IgE antibody to mite
|
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Published in |
International Journal of Nanomedicine, July 2017
|
DOI | 10.2147/ijn.s134539 |
Pubmed ID | |
Authors |
Yan Zhao, Qiang Zhang, Qingfeng Meng, Fenglian Wu, Lihua Zhang, Yao Tang, Yuanyuan Guan, Lixin An |
Abstract |
Semiquantitative and rapid detection of specific IgE (sIgE) with well clinical relevance to house dust mite (HDM) are promising for prevalence rhinitis and asthma patients due to the increasing air pollution. However, the conventional IgE measurement systems are time-consuming, complicated and require special instruments. Herein, we overcome the above limitations of sIgE to HDM detection system by developing a quantum dot nanobeads-based lateral flow immunoassay and an image analysis procedure. The proposed detection system could semiquantitatively measure the IgE in a linear range of 0.2-10 U/mL. Moreover, there is a well correlation between the developed detection system and the clinical symptoms by a comparison study using 56 positive patients' sera and 40 healthy control sera. The proposed detection system is simple, robust and easy-to-use and promising for in home test. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 42 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 6 | 14% |
Student > Master | 5 | 12% |
Student > Ph. D. Student | 5 | 12% |
Student > Bachelor | 3 | 7% |
Lecturer | 2 | 5% |
Other | 3 | 7% |
Unknown | 18 | 43% |
Readers by discipline | Count | As % |
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Agricultural and Biological Sciences | 5 | 12% |
Medicine and Dentistry | 4 | 10% |
Materials Science | 4 | 10% |
Chemistry | 4 | 10% |
Biochemistry, Genetics and Molecular Biology | 2 | 5% |
Other | 4 | 10% |
Unknown | 19 | 45% |