| Title |
Investigation of the effects of carbon-based nanomaterials on A53T alpha-synuclein aggregation using a whole-cell recombinant biosensor
|
|---|---|
| Published in |
International Journal of Nanomedicine, December 2017
|
| DOI | 10.2147/ijn.s144764 |
| Pubmed ID | |
| Authors |
Soheila Mohammadi, Maryam Nikkhah, Saman Hosseinkhani |
| Abstract |
The aggregation of alpha-synuclein (αS), natively unstructured presynaptic protein, is a crucial factor leading to the pathogenesis of Parkinson's disease (PD) and other related disorders. Recent studies have shown prefibrillar and oligomeric intermediates of αS as toxic to the cells. Herein, split-luciferase complementation assay is used to design a "signal-on" biosensor to monitor oligomerization of A53T αS inside the cells. Then, the effect of carbon-based nanomaterials, such as graphene quantum dots (GQDs) and graphene oxide quantum dots (GOQDs), on A53T αS oligomerization in vitro and in living cells is investigated. In this work, for the first time, it was found that GQDs at a concentration of 0.5 μg/mL can promote A53T αS aggregation by shortening the nucleation process, which is the key rate-determining step of fibrillation, thereby making a signal-on biosensor. While these nanomaterials may cross the blood-brain barrier because of their small sizes, the interaction between αS and GQDs may contribute to PD etiology. |
X Demographics
The data shown below were collected from the profiles of 60 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Turkey | 7 | 12% |
| United Kingdom | 1 | 2% |
| Unknown | 52 | 87% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 59 | 98% |
| Unknown | 1 | 2% |
Mendeley readers
The data shown below were compiled from readership statistics for 45 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 45 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 10 | 22% |
| Student > Bachelor | 6 | 13% |
| Student > Master | 6 | 13% |
| Student > Doctoral Student | 3 | 7% |
| Professor | 2 | 4% |
| Other | 0 | 0% |
| Unknown | 18 | 40% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 11 | 24% |
| Agricultural and Biological Sciences | 4 | 9% |
| Engineering | 3 | 7% |
| Chemical Engineering | 1 | 2% |
| Nursing and Health Professions | 1 | 2% |
| Other | 4 | 9% |
| Unknown | 21 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 48. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 01 July 2022.
All research outputs
#901,446
of 25,824,818 outputs
Outputs from International Journal of Nanomedicine
#33
of 4,153 outputs
Outputs of similar age
#19,994
of 447,773 outputs
Outputs of similar age from International Journal of Nanomedicine
#2
of 71 outputs
Altmetric has tracked 25,824,818 research outputs across all sources so far. Compared to these this one has done particularly well and is in the 96th percentile: it's in the top 5% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 4,153 research outputs from this source. They receive a mean Attention Score of 4.2. This one has done particularly well, scoring higher than 99% of its peers.
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We're also able to compare this research output to 71 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 97% of its contemporaries.