| Title |
Antibacterial mechanisms of a novel type picosecond laser-generated silver-titanium nanoparticles and their toxicity to human cells
|
|---|---|
| Published in |
International Journal of Nanomedicine, December 2017
|
| DOI | 10.2147/ijn.s140222 |
| Pubmed ID | |
| Authors |
Peri Korshed, Lin Li, Zhu Liu, Aleksandr Mironov, Tao Wang |
| Abstract |
In this study, we explored the antibacterial mechanisms for a novel type of Ag-TiO2 compound nanoparticles (NPs) produced from an Ag-TiO2 alloy using a picosecond laser and evaluated the toxicity of the Ag-TiO2 NPs to a range of human cell types. Transmission electron microscopy was used to determine the morphology, shapes, and size distribution of the laser-generated Ag-TiO2 NPs. UV-visible spectrometer was used to confirm the shift of light absorbance of the NPs toward visible light wavelength. Results showed that the laser-generated Ag-TiO2 NPs had significant antibacterial activities against both Gram-negative and Gram-positive bacterial strains, including Escherichia coli, Pseudomonas aeruginosa, and the methicillin-resistant Staphylococcus aureus. Increased level of reactive oxygen species was produced by E. coli after exposure to the Ag-TiO2 NPs, which was accompanied with lipid peroxidation, glutathione depletion, disintegration of cell membrane and protein leakage, leading to the cell death. Five types of human cells originated from lung (A549), liver (HePG2), kidney (HEK293), endothelium cells (human coronary artery endothelial cells [hCAECs]), and skin (human dermal fibroblast cells [HDFc]) were used to evaluate the cytotoxicity of the laser-generated Ag-TiO2 NPs. A weak but statistically significant decrease in cell proliferation was observed for hCAECs, A549 and HDFc cells when co-cultured with 2.5 µg/mL or 20 µg/mL of the laser-generated Ag-TiO2 NPs for 48 hours. However, this effect was no longer apparent when a higher concentration of NPs (20 µg/mL) was used after 72 hours of co-culture with human cells, suggesting a possible adaptive process in the cells had occurred. We conclude that picosecond laser-generated Ag-TiO2 NPs have a broad spectrum of antibacterial effect, including against the drug-resistant strain, with multiple underlying molecular mechanisms and low human cell toxicity. The antimicrobial properties of the new type of picoseconds laser-generated Ag-TiO2 compound NPs could have potential biomedical applications. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 34 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 34 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 8 | 24% |
| Researcher | 7 | 21% |
| Student > Ph. D. Student | 7 | 21% |
| Student > Doctoral Student | 3 | 9% |
| Student > Bachelor | 1 | 3% |
| Other | 4 | 12% |
| Unknown | 4 | 12% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 4 | 12% |
| Biochemistry, Genetics and Molecular Biology | 3 | 9% |
| Agricultural and Biological Sciences | 3 | 9% |
| Materials Science | 3 | 9% |
| Engineering | 3 | 9% |
| Other | 11 | 32% |
| Unknown | 7 | 21% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 January 2018.
All research outputs
#22,764,772
of 25,382,440 outputs
Outputs from International Journal of Nanomedicine
#3,598
of 4,122 outputs
Outputs of similar age
#384,359
of 444,941 outputs
Outputs of similar age from International Journal of Nanomedicine
#59
of 71 outputs
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