Title |
Targeting polyelectrolyte networks in purulent body fluids to modulate bactericidal properties of some antibiotics
|
---|---|
Published in |
Infection and Drug Resistance, January 2018
|
DOI | 10.2147/idr.s145337 |
Pubmed ID | |
Authors |
Robert Bucki, Bonita Durnaś, Marzena Wątek, Ewelina Piktel, Katrina Cruz, Przemysław Wolak, Paul B Savage, Paul A Janmey |
Abstract |
The response of the human immune system to most bacterial infections results in accumulation of neutrophils at infection sites that release a significant quantity of DNA and F-actin. Both are negatively charged polyelectrolytes that can interact with positively charged host defense molecules such as cathelicidin-delivered LL-37 peptide or other cationic antibiotic agents. Evaluation of the ability of bacterial outgrowth (using luminescence measurements or counting colony-forming units) to form a biofilm (quantified by crystal violet staining) and analysis of the structure of DNA/F-actin network by optical microscopy in human pus samples treated with different antibiotics in combination with plasma gelsolin, DNAse 1, and/or poly-aspartic acid revealed that bactericidal activity of most tested antibacterial agents increases in the presence of DNA/F-actin depolymerizing factors. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Scientists | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 16 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Other | 4 | 25% |
Researcher | 3 | 19% |
Student > Doctoral Student | 2 | 13% |
Student > Ph. D. Student | 2 | 13% |
Lecturer | 1 | 6% |
Other | 1 | 6% |
Unknown | 3 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Medicine and Dentistry | 5 | 31% |
Pharmacology, Toxicology and Pharmaceutical Science | 4 | 25% |
Immunology and Microbiology | 3 | 19% |
Physics and Astronomy | 1 | 6% |
Unknown | 3 | 19% |