| Title |
A promising magnetic resonance stem cell tracer based on natural biomaterials in a biological system: manganese (II) chelated to melanin nanoparticles
|
|---|---|
| Published in |
International Journal of Nanomedicine, March 2018
|
| DOI | 10.2147/ijn.s157508 |
| Pubmed ID | |
| Authors |
Shi-Jie Liu, Ling-Jie Wang, Ying Qiao, Hua Zhang, Li-Ping Li, Jing-Hua Sun, Sheng He, Wen Xu, Xi Yang, Wen-Wen Cai, Jian-Ding Li, Bin-Quan Wang, Rui-Ping Zhang |
| Abstract |
Melanin and manganese are both indispensable natural substances that play crucial roles in the human body. Melanin has been used as a multimodality imaging nanoplatform for biology science research because of its natural binding ability with metal ions (eg, 64Cu2+, Fe3+, and Gd3+). Because of its effects on T1 signal enhancement, Mn-based nanoparticles have been used in magnetic resonance (MR) quantitative cell tracking in vivo. Stem cell tracking in vivo is an essential technology used to characterize engrafted stem cells, including cellular viability, biodistribution, differentiation capacity, and long-term fate. In the present study, manganese(II) ions chelated to melanin nanoparticles [MNP-Mn(II)] were synthesized. The characteristics, stem cell labeling efficiency, and cytotoxicity of the nanoparticles were evaluated. MR imaging of the labeled stem cells in vivo and in vitro were also further performed. In T1 relaxivity (r1), MNP-Mn(II) were significantly more abundant than Omniscan. Bone marrow-derived stem cells (BMSCs) can be labeled easily by coincubating with MNP-Mn(II), suggesting that MNP-Mn(II) had high biocompatibility. Cell Counting Kit-8 assays revealed that MNP-Mn(II) had almost no cytotoxicity when used to label BMSCs, even with a very high concentration (1,600 µg/mL). BMSCs labeled with MNP-Mn(II) could generate a hyperintense T1 signal both in vitro and in vivo, and the hyperintense T1 signal in vivo persisted for at least 28 days. Taken together, our results showed that MNP-Mn(II) possessed many excellent properties for potential quantitative stem cell tracking in vivo. |
X Demographics
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Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 2 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 17 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 3 | 18% |
| Researcher | 3 | 18% |
| Student > Ph. D. Student | 2 | 12% |
| Student > Postgraduate | 2 | 12% |
| Student > Master | 1 | 6% |
| Other | 1 | 6% |
| Unknown | 5 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 18% |
| Biochemistry, Genetics and Molecular Biology | 2 | 12% |
| Medicine and Dentistry | 2 | 12% |
| Agricultural and Biological Sciences | 1 | 6% |
| Engineering | 1 | 6% |
| Other | 0 | 0% |
| Unknown | 8 | 47% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 12 August 2018.
All research outputs
#19,951,180
of 25,382,440 outputs
Outputs from International Journal of Nanomedicine
#2,971
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Outputs of similar age
#253,777
of 344,853 outputs
Outputs of similar age from International Journal of Nanomedicine
#49
of 81 outputs
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