Title |
Nanoformulation of D-α-tocopheryl polyethylene glycol 1000 succinate-b-poly(ε-caprolactone-ran-glycolide) diblock copolymer for siRNA targeting HIF-1α for nasopharyngeal carcinoma therapy
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Published in |
International Journal of Nanomedicine, February 2015
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DOI | 10.2147/ijn.s76092 |
Pubmed ID | |
Authors |
Yuhan Chen, Gang Xu, Yi Zheng, Maosheng Yan, Zihuang Li, Yayan Zhou, Lin Mei, Xianming Li |
Abstract |
Hypoxia-inducible factor-1α (HIF-1α) is a crucial transcription factor that plays an important role in the carcinogenesis and development of nasopharyngeal carcinoma. In this research, a novel biodegradable D-α-tocopheryl polyethylene glycol 1000 succinate-b-poly(ε-caprolactone-ran-glycolide) (TPGS-b-(PCL-ran-PGA)) nanoparticle (NP) was prepared as a delivery system for small interfering ribonucleic acid (siRNA) molecules targeting HIF-1α in nasopharyngeal carcinoma gene therapy. The results showed that the NPs could efficiently deliver siRNA into CNE-2 cells. CNE-2 cells treated with the HIF-1α siRNA-loaded TPGS-b-(PCL-ran-PGA) NPs showed reduction of HIF-1α expression after 48 hours of incubation via real-time reverse transcriptase-polymerase chain reaction and Western blot analysis. The cytotoxic effect on CNE-2 cells was significantly increased by HIF-1α siRNA-loaded NPs when compared with control groups. In a mouse tumor xenograft model, the HIF-1α siRNA-loaded NPs efficiently suppressed tumor growth, and the levels of HIF-1α mRNA and protein were significantly decreased. These results suggest that TPGS-b-(PCL-ran-PGA) NPs could function as a promising genetic material carrier in antitumor therapy, including therapy for nasopharyngeal carcinoma. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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India | 1 | 5% |
Unknown | 18 | 95% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 6 | 32% |
Student > Bachelor | 4 | 21% |
Other | 2 | 11% |
Student > Doctoral Student | 1 | 5% |
Student > Master | 1 | 5% |
Other | 1 | 5% |
Unknown | 4 | 21% |
Readers by discipline | Count | As % |
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Medicine and Dentistry | 5 | 26% |
Pharmacology, Toxicology and Pharmaceutical Science | 2 | 11% |
Agricultural and Biological Sciences | 2 | 11% |
Immunology and Microbiology | 2 | 11% |
Biochemistry, Genetics and Molecular Biology | 1 | 5% |
Other | 3 | 16% |
Unknown | 4 | 21% |