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Effective small interfering RNA delivery in vitro via a new stearylated cationic peptide

Overview of attention for article published in International Journal of Nanomedicine, May 2015
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Mentioned by

twitter
1 tweeter
facebook
2 Facebook pages

Citations

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11 Dimensions

Readers on

mendeley
17 Mendeley
Title
Effective small interfering RNA delivery in vitro via a new stearylated cationic peptide
Published in
International Journal of Nanomedicine, May 2015
DOI 10.2147/ijn.s79306
Pubmed ID
Authors

Pu Chen, Baoling Chen, Ran Pan, Diana Askhatova

Abstract

A crucial bottleneck in RNA interference-based gene therapy is the lack of safe and efficient delivery systems. Here, a novel small interfering RNA (siRNA) delivery peptide, STR-HK, was constructed by conjugating a stearyl end to the N-terminus of the peptide sequence HHHPKPKRKV, where PKPKRKV is an altered sequence of the nucleus localization signal (PKKKRKV) and contributes to the cytosol localization of STR-HK-siRNA complexes. Histidine is a linker and plays an important role in disrupting the endosomal membrane via the proton sponge effect. As expected, STR-HK formed complexes with siRNA with a particle size of 80-160 nm in diameter and efficiently delivered Cy3-labeled glyceraldehyde 3-phosphate dehydrogenase siRNA into PC-3 human prostate cancer cells. The transfection efficiency of STR-HK at molar ratio of 60/1 was comparable to that of Lipofectamine 2000, one of the most efficient commercially available transfection reagents. Furthermore, the STR-HK-siRNA complexes exhibited minimal cytotoxicity, which was significantly lower than that of Lipofectamine. Taken together, the strategy of conjugating the stearyl moiety with HHHPKPKRKV as a non-viral siRNA delivery system is advantageous.

Twitter Demographics

The data shown below were collected from the profile of 1 tweeter who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 17 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 17 100%

Demographic breakdown

Readers by professional status Count As %
Researcher 4 24%
Student > Bachelor 3 18%
Student > Master 2 12%
Student > Ph. D. Student 2 12%
Professor 1 6%
Other 1 6%
Unknown 4 24%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 24%
Agricultural and Biological Sciences 2 12%
Nursing and Health Professions 1 6%
Pharmacology, Toxicology and Pharmaceutical Science 1 6%
Unspecified 1 6%
Other 2 12%
Unknown 6 35%

Attention Score in Context

This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 January 2016.
All research outputs
#3,468,474
of 7,007,649 outputs
Outputs from International Journal of Nanomedicine
#947
of 1,655 outputs
Outputs of similar age
#109,727
of 202,531 outputs
Outputs of similar age from International Journal of Nanomedicine
#38
of 55 outputs
Altmetric has tracked 7,007,649 research outputs across all sources so far. This one is in the 28th percentile – i.e., 28% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,655 research outputs from this source. They receive a mean Attention Score of 2.1. This one is in the 27th percentile – i.e., 27% of its peers scored the same or lower than it.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 202,531 tracked outputs that were published within six weeks on either side of this one in any source. This one is in the 35th percentile – i.e., 35% of its contemporaries scored the same or lower than it.
We're also able to compare this research output to 55 others from the same source and published within six weeks on either side of this one. This one is in the 10th percentile – i.e., 10% of its contemporaries scored the same or lower than it.