Title |
Magnetic nanoparticles enhance the anticancer activity of cathelicidin LL-37 peptide against colon cancer cells
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Published in |
International Journal of Nanomedicine, June 2015
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DOI | 10.2147/ijn.s76104 |
Pubmed ID | |
Authors |
Katarzyna Niemirowicz, Izabela Prokop, Agnieszka Z Wilczewska, Urszula Wnorowska, Ewelina Piktel, Marzena Wątek, Paul B Savage, Robert Bucki |
Abstract |
The pleiotropic activity of human cathelicidin LL-37 peptide includes an ability to suppress development of colon cancer cells. We hypothesized that the anticancer activity of LL-37 would improve when attached to the surface of magnetic nanoparticles (MNPs). Using colon cancer culture (DLD-1 cells and HT-29 cells), we evaluated the effects of MNPs, LL-37 peptide, its synthetic analog ceragenin CSA-13, and two novel nanosystems, ie, MNP@LL-37 and MNP@CSA-13, on cancer cell viability and apoptosis. Treatment of cancer cells with the LL-37 peptide linked to MNPs (MNP@LL-37) caused a greater decrease in cell viability and a higher rate of apoptosis compared with treatment using free LL-37 peptide. Additionally, we observed a strong ability of ceragenin CSA-13 and MNP@CSA-13 to induce apoptosis of DLD-1 cells. We found that both nanosystems were successfully internalized by HT-29 cells, and cathelicidin LL-37 and ceragenin CSA-13 might play a key role as novel homing molecules. These results indicate that the previously described anticancer activity of LL-37 peptide against colon cancer cells might be significantly improved using a theranostic approach. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Canada | 1 | 25% |
Unknown | 3 | 75% |
Demographic breakdown
Type | Count | As % |
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Practitioners (doctors, other healthcare professionals) | 3 | 75% |
Members of the public | 1 | 25% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
United States | 2 | 3% |
Singapore | 1 | 1% |
Unknown | 64 | 96% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 10 | 15% |
Student > Bachelor | 10 | 15% |
Researcher | 8 | 12% |
Student > Master | 8 | 12% |
Other | 4 | 6% |
Other | 8 | 12% |
Unknown | 19 | 28% |
Readers by discipline | Count | As % |
---|---|---|
Chemistry | 9 | 13% |
Biochemistry, Genetics and Molecular Biology | 9 | 13% |
Agricultural and Biological Sciences | 5 | 7% |
Pharmacology, Toxicology and Pharmaceutical Science | 5 | 7% |
Immunology and Microbiology | 4 | 6% |
Other | 13 | 19% |
Unknown | 22 | 33% |