| Title |
Molecular characterization of carbapenem-resistant Gram-negative bacilli clinical isolates in Algeria
|
|---|---|
| Published in |
Infection and Drug Resistance, May 2018
|
| DOI | 10.2147/idr.s150005 |
| Pubmed ID | |
| Authors |
Nadjette Bourafa, Wafaa Chaalal, Sofiane Bakour, Rym Lalaoui, Nafissa Boutefnouchet, Seydina M Diene, Jean-Marc Rolain |
| Abstract |
The aims of this study were to investigate the occurrence of carbapenem-resistant Gram-negative bacilli (GNB) isolated from inpatients and outpatients in Algeria between July and September 2015, and to screen their resistance mechanisms and genetic relatedness. A total of 68 non-redundant isolates were identified using matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF) antibiotic susceptibility testing was performed using disk diffusion and Etest methods. Carbapenemase activity was carried out using modified Carba NP test, EDTA assay, and the modified Hodge test. Molecular characterization of carbapenemases and extended-spectrum β-lactamase (ESBL) genes were detected by standard PCR and sequencing. Genotyping of carbapenem-resistant isolates was performed by multilocus sequence typing (MLST) analysis. Of the 68 GNB isolates, 13 (19%) showed reduced susceptibility to carbapenems, including, four Klebsiella pneumoniae, one Escherichia coli, six Acinetobacter baumannii, and two Pseudomonas aeruginosa. blaOXA-48 gene was detected in the five Enterobacteriaceae isolates, and blaOXA-23 was identified in all A. baumannii isolates. OprD mutations were revealed in the two P. aeruginosa isolates. A total of 11 out of the 13 carbapenem-resistant GNB were detected in inpatients, and the two remaining strains were isolated from outpatients. Molecular typing showed the presence of four sequence types (STs) among the OXA-48-producing K. pneumoniae isolates: ST101, ST147, ST163, and ST2017. ST533 was identified for the OXA-48 producing E. coli isolate. All of the A. baumannii and P. aeruginosa were assigned to the international clonal lineages ST2 and ST654, respectively. This study reports the first detection of the epidemic multidrug-resistant lineage, K. pneumoniae ST147 coproduced blaOXA-48 and ESBL genes in Algeria and represents the first description of OXA-48-producing E. coli ST533 and K. pneumoniae ST163 and ST2017. In addition, this study describes for the first time the emergence of OXA-48-producing E. coli and K. pneumoniae in the community in Algeria, leading to major problems for managing microbial infections. |
X Demographics
The data shown below were collected from the profiles of 6 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| France | 1 | 17% |
| Australia | 1 | 17% |
| Unknown | 4 | 67% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 6 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 77 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 77 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 17 | 22% |
| Researcher | 10 | 13% |
| Student > Master | 5 | 6% |
| Lecturer | 4 | 5% |
| Student > Doctoral Student | 4 | 5% |
| Other | 13 | 17% |
| Unknown | 24 | 31% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 17 | 22% |
| Biochemistry, Genetics and Molecular Biology | 10 | 13% |
| Medicine and Dentistry | 7 | 9% |
| Veterinary Science and Veterinary Medicine | 5 | 6% |
| Agricultural and Biological Sciences | 5 | 6% |
| Other | 6 | 8% |
| Unknown | 27 | 35% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 January 2020.
All research outputs
#12,887,660
of 23,056,273 outputs
Outputs from Infection and Drug Resistance
#343
of 1,691 outputs
Outputs of similar age
#153,880
of 326,183 outputs
Outputs of similar age from Infection and Drug Resistance
#7
of 32 outputs
Altmetric has tracked 23,056,273 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,691 research outputs from this source. They receive a mean Attention Score of 3.9. This one has done well, scoring higher than 79% of its peers.
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We're also able to compare this research output to 32 others from the same source and published within six weeks on either side of this one. This one has done well, scoring higher than 78% of its contemporaries.