| Title |
Exploration of peptide T7 and its derivative as integrin αvβ3-targeted imaging agents
|
|---|---|
| Published in |
OncoTargets and therapy, June 2015
|
| DOI | 10.2147/ott.s82095 |
| Pubmed ID | |
| Authors |
Xin He, Yumei Hao, Wei Long, Naling Song, Saijun Fan, Aimin Meng |
| Abstract |
The aim of the present study was to develop potential candidates of integrin αvβ3-targeted imaging agent, which can facilitate the diagnosis and treatment of malignant solid tumors. Peptides derived from tumstatin, named T7 and T7-6H, were derivatized to contain histidine in the C-terminus of their sequence and were labeled with (99m)Tc via nitrido and carbonyl precursors. The radiochemical purity and stability of (99m)Tc-labeled T7 and T7-6H were characterized by thin-layer chromatography. The whole body biodistribution was studied in NCI-H157-bearing BALB/c nude mice. The (99m)Tc-labeled T7 and T7-6H showed adequate in vitro stability, with a high radiochemical purity of over 90%. The dissociation constant (Kd) value of the (99m)Tc-labeled T7 and T7-6H ranged from 68.5 nM to 140.8 nM in U251 and NCI-H157 cell lines. (99m)Tc-labeled T7 and T7-6H showed no significant difference of biodistribution in mice. Furthermore, both T7 and T7-6H exhibited a poor blood-brain barrier penetration and a transient accumulation in lung; the uptake in tumor tissues was significantly higher than in muscle tissue, with a ratio of 5.8. (99m)Tc-labeled T7 and T7-6H can be regarded as promising single-photon emission computed tomography probes for imaging integrin αvβ3, and need to be further studied for noninvasive detection of tumors. |
Mendeley readers
The data shown below were compiled from readership statistics for 13 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 13 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 2 | 15% |
| Student > Bachelor | 2 | 15% |
| Professor > Associate Professor | 1 | 8% |
| Student > Postgraduate | 1 | 8% |
| Unknown | 7 | 54% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 2 | 15% |
| Chemical Engineering | 1 | 8% |
| Mathematics | 1 | 8% |
| Agricultural and Biological Sciences | 1 | 8% |
| Medicine and Dentistry | 1 | 8% |
| Other | 0 | 0% |
| Unknown | 7 | 54% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 17 June 2015.
All research outputs
#17,302,400
of 25,394,764 outputs
Outputs from OncoTargets and therapy
#1,149
of 3,016 outputs
Outputs of similar age
#168,600
of 281,494 outputs
Outputs of similar age from OncoTargets and therapy
#28
of 71 outputs
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