| Title |
Molecular study of wound healing after using biosynthesized BNC/Fe3O4 nanocomposites assisted with a bioinformatics approach
|
|---|---|
| Published in |
International Journal of Nanomedicine, May 2018
|
| DOI | 10.2147/ijn.s159637 |
| Pubmed ID | |
| Authors |
Mona Moniri, Amin Boroumand Moghaddam, Susan Azizi, Raha Abdul Rahim, Wan Zuhainis Saad, Mohammad Navaderi, Palanisamy Arulselvan, Rosfarizan Mohamad |
| Abstract |
Molecular investigation of wound healing has allowed better understanding about interaction of genes and pathways involved in healing progression. The aim of this study was to prepare magnetic/bacterial nanocellulose (Fe3O4/BNC) nanocomposite films as ecofriendly wound dressing in order to evaluate their physical, cytotoxicity and antimicrobial properties. The molecular study was carried out to evaluate expression of genes involved in healing of wounds after treatment with BNC/Fe3O4 films. Magnetic nanoparticles were biosynthesized by using Aloe vera extract in new isolated bacterial nanocellulose (BNC) RM1. The nanocomposites were characterized using X-ray diffraction, Fourier transform infrared, and field emission scanning electron microscopy. Moreover, swelling property and metal ions release profile of the nanocomposites were investigated. The ability of nanocomposites to promote wound healing of human dermal fibroblast cells in vitro was examined. Bioinformatics databases were used to identify genes with important healing effect. Key genes which interfered with healing were studied by quantitative real time PCR. Spherical magnetic nanoparticles (15-30 nm) were formed and immobilized within the structure of BNC. The BNC/Fe3O4 was nontoxic (IC50>500 μg/mL) with excellent wound healing efficiency after 48 hours. The nanocomposites showed good antibacterial activity ranging from 6±0.2 to 13.40±0.10 mm against Staphylococcus aureus, Staphylococcus epidermidis and Pseudomonas aeruginosa. The effective genes for the wound healing process were TGF-B1, MMP2, MMP9, Wnt4, CTNNB1, hsa-miR-29b, and hsa-miR-29c with time dependent manner. BNC/Fe3O4 has an effect on microRNA by reducing its expression and therefore causing an increase in the gene expression of other genes, which consequently resulted in wound healing. This eco-friendly nanocomposite with excellent healing properties can be used as an effective wound dressing for treatment of cutaneous wounds. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Malaysia | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 97 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 97 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Master | 15 | 15% |
| Student > Bachelor | 12 | 12% |
| Researcher | 10 | 10% |
| Student > Ph. D. Student | 9 | 9% |
| Professor > Associate Professor | 6 | 6% |
| Other | 17 | 18% |
| Unknown | 28 | 29% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 10 | 10% |
| Agricultural and Biological Sciences | 8 | 8% |
| Medicine and Dentistry | 8 | 8% |
| Materials Science | 6 | 6% |
| Pharmacology, Toxicology and Pharmaceutical Science | 6 | 6% |
| Other | 20 | 21% |
| Unknown | 39 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 15 October 2021.
All research outputs
#16,728,456
of 25,382,440 outputs
Outputs from International Journal of Nanomedicine
#2,087
of 4,122 outputs
Outputs of similar age
#208,374
of 339,234 outputs
Outputs of similar age from International Journal of Nanomedicine
#44
of 70 outputs
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