Title |
Low claudin-6 expression correlates with poor prognosis in patients with non-small cell lung cancer
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Published in |
OncoTargets and therapy, July 2015
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DOI | 10.2147/ott.s85478 |
Pubmed ID | |
Authors |
Qiang Wang, Yan Zhang, Tao Zhang, Zhi-Gang Han, Li Shan |
Abstract |
Claudins are found in junctional complexes mediating cell adhesion and are involved in the attachment of tight junctions to the underlying cytoskeleton. Abnormal claudin-6 expression has been observed for a variety of malignant solid tumors, but the expression of claudin-6 in non-small cell lung cancer (NSCLC) has not yet been characterized. Immunohistochemistry, reverse transcription-polymerase chain reaction (RT-PCR), and western blot analysis were used to quantify claudin-6 expression in 123 cases of NSCLC and non-cancerous adjacent tissue. We analyzed the relationship between claudin-6 expression and clinicopathological features of NSCLC. The Kaplan-Meier method was used to analyze postoperative survival rates, and the log-rank test was used to assess differences in survival rates. The Cox regression model was used to perform multivariate analysis. Claudin-6 expression was low for 61 of 123 (49.6%) NSCLC tissue samples and for 33 of 123 (26.8%) normal adjacent tissue samples. RT-PCR and western blot analyses confirmed the immunohistochemistry results. Claudin-6 expression was associated with lymph node metastasis (P<0.001) and TNM stage (P=0.007). Kaplan-Meier analysis indicated that patients with low claudin-6 expression had significantly lower survival rates than those with high claudin-6 expression. Multivariate analysis suggested that low claudin-6 expression was an independent indicator of prognosis in NSCLC patients. Low claudin-6 expression is an independent prognostic biomarker that indicates a worse prognosis in patients with NSCLC. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 13 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Bachelor | 2 | 15% |
Student > Ph. D. Student | 2 | 15% |
Student > Master | 2 | 15% |
Other | 1 | 8% |
Professor | 1 | 8% |
Other | 3 | 23% |
Unknown | 2 | 15% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 2 | 15% |
Medicine and Dentistry | 2 | 15% |
Immunology and Microbiology | 2 | 15% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 8% |
Agricultural and Biological Sciences | 1 | 8% |
Other | 2 | 15% |
Unknown | 3 | 23% |