Title |
Obesity-associated miR-27a upregulation promotes hepatocellular carcinoma metastasis through suppressing SFRP1
|
---|---|
Published in |
OncoTargets and therapy, June 2018
|
DOI | 10.2147/ott.s162978 |
Pubmed ID | |
Authors |
Yang Chen, Fan Zhang, Yawei Zhao, Kan He, Xiao Zheng, Yue Pan, Dan Shao, Pei Shang, Yongsheng Yang, Dan Zhang, Yingjun Xie, Xiaoxiao Yao, Li Chen, Jing Li, Xuewen Zhang |
Abstract |
Obesity was a recognized risk factor for the development and progression of hepatocellular carcinoma (HCC). However, the effects and mechanisms by which obesity promotes HCC metastasis remain poorly understood. We cultured adipocyte induced by preadipocyte 3T3-L1 in vitro and established HCC metastasis model in obesity mouse in vivo to mimic the tumor microenvironment in obese status. The mechanisms underlying obesity-associated miR-27a upregulation promoting HCC metastasis were investigated. In this study, we showed that miR-27a was upregulated in adipocytes, obese mouse model and clinical samples, and the increased miR-27a level promoted migration and invasion in HCC cells, increased the number of metastasis nodes in obese mouse model, and was associated with poor clinical outcomes. Overexpressed secreted frizzled-related protein 1 in HCC cells and tissues significantly alleviated the upregulation of β-catenin and matrix metalloproteinase-7 induced by high level of miR-27a. Meanwhile, the E-cadherin expression decreased and Vimentin expression increased, linking with high level of β-catenin in high-fat group. Taken together, our results have elucidated the critical role of extracellular miR-27a as a pro-metastatic factor in HCC and revealed that obesity-associated miR-27a upregulation promoted HCC metastasis through activated Wnt/β-catenin signaling by suppressing secreted frizzled-related protein 1. Our findings shed light on the novel mechanism underlying HCC metastasis and provided miR-27a as a promising target for obese liver cancer therapy. |
X Demographics
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 1 | 100% |
Demographic breakdown
Type | Count | As % |
---|---|---|
Members of the public | 1 | 100% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 21 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Researcher | 4 | 19% |
Student > Ph. D. Student | 3 | 14% |
Student > Master | 3 | 14% |
Student > Bachelor | 3 | 14% |
Unspecified | 1 | 5% |
Other | 2 | 10% |
Unknown | 5 | 24% |
Readers by discipline | Count | As % |
---|---|---|
Biochemistry, Genetics and Molecular Biology | 7 | 33% |
Unspecified | 1 | 5% |
Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
Agricultural and Biological Sciences | 1 | 5% |
Immunology and Microbiology | 1 | 5% |
Other | 2 | 10% |
Unknown | 8 | 38% |