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Cationic polyelectrolyte-mediated delivery of antisense morpholino oligonucleotides for exon-skipping in vitro and in mdx mice

Overview of attention for article published in International Journal of Nanomedicine, September 2015
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About this Attention Score

  • Good Attention Score compared to outputs of the same age (71st percentile)
  • High Attention Score compared to outputs of the same age and source (91st percentile)

Mentioned by

twitter
6 tweeters

Citations

dimensions_citation
11 Dimensions

Readers on

mendeley
15 Mendeley
Title
Cationic polyelectrolyte-mediated delivery of antisense morpholino oligonucleotides for exon-skipping in vitro and in mdx mice
Published in
International Journal of Nanomedicine, September 2015
DOI 10.2147/ijn.s89910
Pubmed ID
Authors

Mingxing Wang, Bo Wu, Jason D. Tucker, Peijuan Lu, Qilong Lu

Abstract

In this study, we investigated a series of cationic polyelectrolytes (PEs) with different size and composition for their potential to improve delivery of an antisense phosphorodiamidate morpholino oligomer (PMO) both in vitro and in vivo. The results showed that the poly(diallyldimethylammonium chloride) (PDDAC) polymer series, especially PE-3 and PE-4, improves the delivery efficiency of PMO, comparable with Endoporter-mediated PMO delivery in vitro. The enhanced PMO delivery and targeting to dystrophin exon 23 was further observed in mdx mice, up to fourfold with the PE-4, compared with PMO alone. The cytotoxicity of the PEs was lower than that of Endoporter and polyethylenimine 25,000 Da in vitro, and was not clearly detected in muscle in vivo under the tested concentrations. Together, these results demonstrate that optimization of PE molecular size, composition, and distribution of cationic charge are key factors to achieve enhanced PMO exon-skipping efficiency. The increased efficiency and lower toxicity show this PDDAC series to be capable gene/antisense oligonucleotide delivery-enhancing agents for treating muscular dystrophy and other diseases.

Twitter Demographics

The data shown below were collected from the profiles of 6 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 15 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
United States 1 7%
Sweden 1 7%
Unknown 13 87%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 3 20%
Student > Doctoral Student 2 13%
Researcher 2 13%
Professor 1 7%
Student > Bachelor 1 7%
Other 3 20%
Unknown 3 20%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 3 20%
Agricultural and Biological Sciences 3 20%
Neuroscience 2 13%
Medicine and Dentistry 1 7%
Chemistry 1 7%
Other 1 7%
Unknown 4 27%

Attention Score in Context

This research output has an Altmetric Attention Score of 4. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 September 2016.
All research outputs
#2,947,586
of 11,339,688 outputs
Outputs from International Journal of Nanomedicine
#269
of 2,260 outputs
Outputs of similar age
#65,810
of 237,767 outputs
Outputs of similar age from International Journal of Nanomedicine
#11
of 148 outputs
Altmetric has tracked 11,339,688 research outputs across all sources so far. This one has received more attention than most of these and is in the 73rd percentile.
So far Altmetric has tracked 2,260 research outputs from this source. They receive a mean Attention Score of 3.0. This one has done well, scoring higher than 87% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 237,767 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 71% of its contemporaries.
We're also able to compare this research output to 148 others from the same source and published within six weeks on either side of this one. This one has done particularly well, scoring higher than 91% of its contemporaries.