| Title |
Influence of cationic lipid concentration on properties of lipid–polymer hybrid nanospheres for gene delivery
|
|---|---|
| Published in |
International Journal of Nanomedicine, September 2015
|
| DOI | 10.2147/ijn.s87120 |
| Pubmed ID | |
| Authors |
Rajendran JC Bose, Yoshie Arai, Jong Chan Ahn, Hansoo Park, Soo-Hong Lee |
| Abstract |
Nanoparticles have been widely used for nonviral gene delivery. Recently, cationic hybrid nanoparticles consisting of two different materials were suggested as a promising delivery vehicle. In this study, nanospheres with a poly(d,l-lactic-co-glycolic acid) (PLGA) core and cationic lipid shell were prepared, and the effect of cationic lipid concentrations on the properties of lipid polymer hybrid nanocarriers investigated. Lipid-polymer hybrid nanospheres (LPHNSs) were fabricated by the emulsion-solvent evaporation method using different concentrations of cationic lipids and characterized for size, surface charge, stability, plasmid DNA-binding capacity, cytotoxicity, and transfection efficiency. All LPHNSs had narrow size distribution with positive surface charges (ζ-potential 52-60 mV), and showed excellent plasmid DNA-binding capacity. In vitro cytotoxicity measurements with HEK293T, HeLa, HaCaT, and HepG2 cells also showed that LPHNSs exhibited less cytotoxicity than conventional transfection agents, such as Lipofectamine and polyethyleneimine-PLGA. As cationic lipid concentrations increased, the particle size of LPHNSs decreased while their ζ-potential increased. In addition, the in vitro transfection efficiency of LPHNSs increased as lipid concentration increased. |
Mendeley readers
The data shown below were compiled from readership statistics for 167 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| United Kingdom | 1 | <1% |
| Unknown | 166 | 99% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 12 | 7% |
| Researcher | 8 | 5% |
| Student > Bachelor | 6 | 4% |
| Student > Doctoral Student | 3 | 2% |
| Student > Postgraduate | 3 | 2% |
| Other | 9 | 5% |
| Unknown | 126 | 75% |
| Readers by discipline | Count | As % |
|---|---|---|
| Pharmacology, Toxicology and Pharmaceutical Science | 10 | 6% |
| Biochemistry, Genetics and Molecular Biology | 8 | 5% |
| Chemistry | 8 | 5% |
| Medicine and Dentistry | 5 | 3% |
| Engineering | 3 | 2% |
| Other | 6 | 4% |
| Unknown | 127 | 76% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 05 March 2024.
All research outputs
#8,618,268
of 25,582,611 outputs
Outputs from International Journal of Nanomedicine
#1,077
of 4,077 outputs
Outputs of similar age
#95,150
of 277,177 outputs
Outputs of similar age from International Journal of Nanomedicine
#37
of 153 outputs
Altmetric has tracked 25,582,611 research outputs across all sources so far. This one is in the 43rd percentile – i.e., 43% of other outputs scored the same or lower than it.
So far Altmetric has tracked 4,077 research outputs from this source. They receive a mean Attention Score of 4.8. This one has gotten more attention than average, scoring higher than 63% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 277,177 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 55% of its contemporaries.
We're also able to compare this research output to 153 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 73% of its contemporaries.