Title |
Polyethyleneimine-functionalized boron nitride nanospheres as efficient carriers for enhancing the immunostimulatory effect of CpG oligodeoxynucleotides
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Published in |
International Journal of Nanomedicine, August 2015
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DOI | 10.2147/ijn.s88774 |
Pubmed ID | |
Authors |
Huijie Zhang, Shini Feng, Ting Yan, Chunyi Zhi, Xiao-Dong Gao, Nobutaka Hanagata |
Abstract |
CpG oligodeoxynucleotides (ODNs) stimulate innate and adaptive immune responses. Thus, these molecules are promising therapeutic agents and vaccine adjuvants against various diseases. In this study, we developed a novel CpG ODNs delivery system based on polyethyleneimine (PEI)-functionalized boron nitride nanospheres (BNNS). PEI was coated on the surface of BNNS via electrostatic interactions. The prepared BNNS-PEI complexes had positive zeta potential and exhibited enhanced dispersity and stability in aqueous solution. In vitro cytotoxicity assays revealed that the BNNS-PEI complexes with concentrations up to 100 μg/mL exhibited no obvious cytotoxicity. Furthermore, the positively charged surface of the BNNS-PEI complexes greatly improved the loading capacity and cellular uptake efficiency of CpG ODNs. Class B CpG ODNs loaded on the BNNS-PEI complexes enhanced the production of interleukin-6 and tumor necrosis factor-α from peripheral blood mononuclear cells compared with CpG ODNs directly loaded on BNNS. Contrary to the free CpG ODNs or CpG ODNs directly loaded on BNNS, class B CpG ODNs loaded on the BNNS-PEI complexes induced interferon-α simultaneously. PEI coating may have changed the physical form of class B CpG ODNs on BNNS, which further affected their interaction with Toll-like receptor 9 and induced interferon-α. Therefore, BNNS-PEI complexes can be used to enhance the immunostimulatory effect and therapeutic activity of CpG ODNs and the treatment of diseases requiring interleukin-6, tumor necrosis factor-α, and interferon-α. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
India | 1 | 3% |
Unknown | 35 | 97% |
Demographic breakdown
Readers by professional status | Count | As % |
---|---|---|
Student > Ph. D. Student | 7 | 19% |
Professor > Associate Professor | 4 | 11% |
Student > Doctoral Student | 4 | 11% |
Researcher | 3 | 8% |
Student > Bachelor | 2 | 6% |
Other | 9 | 25% |
Unknown | 7 | 19% |
Readers by discipline | Count | As % |
---|---|---|
Materials Science | 9 | 25% |
Chemical Engineering | 3 | 8% |
Pharmacology, Toxicology and Pharmaceutical Science | 3 | 8% |
Biochemistry, Genetics and Molecular Biology | 3 | 8% |
Medicine and Dentistry | 3 | 8% |
Other | 6 | 17% |
Unknown | 9 | 25% |