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Delivery of DNAzyme targeting aurora kinase A to inhibit the proliferation and migration of human prostate cancer

Overview of attention for article published in International Journal of Nanomedicine, September 2015
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About this Attention Score

  • Above-average Attention Score compared to outputs of the same age (59th percentile)
  • Good Attention Score compared to outputs of the same age and source (72nd percentile)

Mentioned by

twitter
5 tweeters

Citations

dimensions_citation
27 Dimensions

Readers on

mendeley
22 Mendeley
Title
Delivery of DNAzyme targeting aurora kinase A to inhibit the proliferation and migration of human prostate cancer
Published in
International Journal of Nanomedicine, September 2015
DOI 10.2147/ijn.s90559
Pubmed ID
Authors

Quanshun Li, Zhen Xing, Sai Gao, Yan Duan, Haobo Han, Li, Yan Yang

Abstract

Herein, a polyethylenimine derivative N-acetyl-l-leucine-polyethylenimine (N-Ac-l-Leu-PEI) was employed as a carrier to achieve the delivery of DNAzyme targeting aurora kinase A using PC-3 cell as a model. Flow cytometry and confocal laser scanning microscopy demonstrated that the derivative could realize the cellular uptake of nanoparticles in an energy-dependent and clathrin-mediated pathway and obtain a high DNAzyme concentration in the cytoplasm through further endosomal escape. After DNAzyme transfection, expression level of aurora kinase A would be downregulated at the protein level. Meanwhile, the inhibition of cell proliferation was observed through 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide and cell colony formation assay, attributing to the activation of apoptosis and cell cycle arrest. Through flow cytometric analysis, an early apoptotic ratio of 25.93% and G2 phase of 22.58% has been detected after N-Ac-l-Leu-PEI-mediated DNAzyme transfection. Finally, wound healing and Transwell migration assay showed that DNAzyme transfection could efficiently inhibit the cell migration. These results demonstrated that N-Ac-l-Leu-PEI could successfully mediate the DNAzyme delivery and downregulate the expression level of aurora kinase A, triggering a significant inhibitory effect of excessive proliferation and migration of tumor cells.

Twitter Demographics

The data shown below were collected from the profiles of 5 tweeters who shared this research output. Click here to find out more about how the information was compiled.

Mendeley readers

The data shown below were compiled from readership statistics for 22 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Unknown 22 100%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 4 18%
Researcher 4 18%
Other 3 14%
Student > Bachelor 3 14%
Student > Master 2 9%
Other 3 14%
Unknown 3 14%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 4 18%
Medicine and Dentistry 4 18%
Pharmacology, Toxicology and Pharmaceutical Science 2 9%
Agricultural and Biological Sciences 2 9%
Materials Science 2 9%
Other 3 14%
Unknown 5 23%

Attention Score in Context

This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 11 September 2015.
All research outputs
#2,326,172
of 6,327,010 outputs
Outputs from International Journal of Nanomedicine
#306
of 1,515 outputs
Outputs of similar age
#75,247
of 194,289 outputs
Outputs of similar age from International Journal of Nanomedicine
#31
of 147 outputs
Altmetric has tracked 6,327,010 research outputs across all sources so far. This one has received more attention than most of these and is in the 61st percentile.
So far Altmetric has tracked 1,515 research outputs from this source. They receive a mean Attention Score of 1.8. This one has done well, scoring higher than 76% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 194,289 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 59% of its contemporaries.
We're also able to compare this research output to 147 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.