| Title |
Identification of novel target genes in human lung tissue involved in chronic obstructive pulmonary disease
|
|---|---|
| Published in |
International Journal of Chronic Obstructive Pulmonary Disease, July 2018
|
| DOI | 10.2147/copd.s161958 |
| Pubmed ID | |
| Authors |
Lena Heinbockel, Sebastian Marwitz, Andra B Schromm, Henrik Watz, Christian Kugler, Ole Ammerpohl, Karoline Schnepf, Klaus F Rabe, Daniel Droemann, Torsten Goldmann |
| Abstract |
As part of a study aimed at illuminating at least some of the complex molecular events taking place in COPD, we screened tissues by means of transcriptome analyses. Tissues were subjected to transcriptome analysis. Candidate genes were identified and validated by immunohistochemistry. Primary human lung cells were subjected to stimulation with cigarette smoke extract for further validation by real time PCR. Six candidate genes were selected for further investigations: Aquaporin 3 (AQP3), extracellular matrix protein 1 (ECM1), four and a half LIM domain 1 (FHL1), milk fat globule epidermal growth factor 8 (MFGE8, lactadherin), phosphodiesterase 4D-interacting protein (PDE4DIP), and creatine transporter SLC6A8. All six proteins were allocated to distinct cell types by immunohistochemistry. Upon stimulation with cigarette smoke extract, human type II pneumocytes showed a dose-dependent down-regulation of MFGE8, while ECM1 and FHL1 also tended to be down-regulated. Although present, none of the candidates was regulated by cigarette smoke extract in primary human macrophages. MFGE8 turned out to be an interesting new candidate gene in COPD deserving further studies. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 21 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 21 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 7 | 33% |
| Student > Ph. D. Student | 3 | 14% |
| Lecturer | 1 | 5% |
| Student > Bachelor | 1 | 5% |
| Other | 1 | 5% |
| Other | 3 | 14% |
| Unknown | 5 | 24% |
| Readers by discipline | Count | As % |
|---|---|---|
| Medicine and Dentistry | 4 | 19% |
| Biochemistry, Genetics and Molecular Biology | 3 | 14% |
| Agricultural and Biological Sciences | 3 | 14% |
| Immunology and Microbiology | 3 | 14% |
| Pharmacology, Toxicology and Pharmaceutical Science | 1 | 5% |
| Other | 0 | 0% |
| Unknown | 7 | 33% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 14 August 2018.
All research outputs
#20,663,600
of 25,385,509 outputs
Outputs from International Journal of Chronic Obstructive Pulmonary Disease
#2,079
of 2,578 outputs
Outputs of similar age
#266,174
of 341,606 outputs
Outputs of similar age from International Journal of Chronic Obstructive Pulmonary Disease
#60
of 72 outputs
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