| Title |
Antibiotic resistance pattern and molecular characterization of extended-spectrum β-lactamase producing enteroaggregative Escherichia coli isolates in children from southwest Iran
|
|---|---|
| Published in |
Infection and Drug Resistance, August 2018
|
| DOI | 10.2147/idr.s167271 |
| Pubmed ID | |
| Authors |
Mansour Amin, Mehrandokht Sirous, Hazhir Javaherizadeh, Mohammad Motamedifar, Morteza Saki, Hojat Veisi, Saeedeh Ebrahimi, Sakineh Seyed-Mohammadi, Mohammad Hashemzadeh |
| Abstract |
Enteroaggregative Escherichia coli (EAEC) has been implicated as an emerging cause of traveler's diarrhea, persistent diarrhea among children, and immunocompromised patients. The present study aimed to investigate the prevalence of antibiotic resistance, extendedspectrum β-lactamase (ESBL) production, and virulence factors of EAEC isolates obtained from Iranian children suffered from diarrhea. In this cross-sectional study, from March 2015 to February 2016, 32 EAEC isolates were collected from fecal samples of children aged <12 years with diarrhea in southwest of Iran. All EAEC isolates identified using phenotypic and molecular methods and the cell line adhesion assay. Antimicrobial susceptibility testing was determined using disk diffusion method. The presence of virulence factors and ESBL resistance genes were determined by polymerase chain reaction. Overall, 28.1% (9/32) of the isolates were positive for at least one of virulence genes. The most frequent gene was aap with a frequency of 96.9%. Neither aafA nor aggA gene was detected among all of the EAEC isolates. Antimicrobial susceptibility testing revealed the highest resistance rate to ampicillin (100%) and co-trimoxazole (100%), followed by ceftriaxone (81.3%). Further analysis revealed that the rate of ESBLs-producing isolates was 71.9% (23/32). Polymerase chain reaction screening revealed that 87.5% and 65.5% of EAEC isolates were positive for blaTEM and blaCTX-M genes, respectively, and 17 (53.1%) of isolates contained both blaTEM and blaCTX-M genes. The high detection rate of ESBL-producing EAEC isolates accompanied with virulence genes highlights a need to restrict infection control policies in order to prevent further dissemination of the resistant and virulent EAEC strains. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Iran, Islamic Republic of | 1 | 50% |
| Unknown | 1 | 50% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 61 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 61 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 12 | 20% |
| Student > Master | 11 | 18% |
| Student > Bachelor | 5 | 8% |
| Student > Ph. D. Student | 5 | 8% |
| Student > Postgraduate | 3 | 5% |
| Other | 9 | 15% |
| Unknown | 16 | 26% |
| Readers by discipline | Count | As % |
|---|---|---|
| Immunology and Microbiology | 10 | 16% |
| Biochemistry, Genetics and Molecular Biology | 7 | 11% |
| Medicine and Dentistry | 6 | 10% |
| Nursing and Health Professions | 4 | 7% |
| Environmental Science | 4 | 7% |
| Other | 12 | 20% |
| Unknown | 18 | 30% |
Attention Score in Context
This research output has an Altmetric Attention Score of 3. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 25 August 2018.
All research outputs
#13,387,978
of 23,099,576 outputs
Outputs from Infection and Drug Resistance
#397
of 1,698 outputs
Outputs of similar age
#164,478
of 331,041 outputs
Outputs of similar age from Infection and Drug Resistance
#21
of 57 outputs
Altmetric has tracked 23,099,576 research outputs across all sources so far. This one is in the 41st percentile – i.e., 41% of other outputs scored the same or lower than it.
So far Altmetric has tracked 1,698 research outputs from this source. They receive a mean Attention Score of 3.8. This one has done well, scoring higher than 75% of its peers.
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We're also able to compare this research output to 57 others from the same source and published within six weeks on either side of this one. This one has gotten more attention than average, scoring higher than 61% of its contemporaries.