| Title |
Biophysical, docking, and cellular studies on the effects of cerium oxide nanoparticles on blood components: in vitro
|
|---|---|
| Published in |
International Journal of Nanomedicine, August 2018
|
| DOI | 10.2147/ijn.s172162 |
| Pubmed ID | |
| Authors |
Neda Eskandari, Mohammad Mahdi Nejadi Babadaei, Sanaz Nikpur, Ghazal Ghasrahmad, Farnoosh Attar, Masoumeh Heshmati, Keivan Akhtari, Seyed Mahdi Rezayat Sorkhabadi, Seyyedeh Elaheh Mousavi, Mojtaba Falahati |
| Abstract |
The application of nanoparticles (NPs) in medicine and biology has received great interest due to their novel features. However, their adverse effects on the biological system are not well understood. This study aims to evaluate the effect of cerium oxide nanoparticles (CNPs) on conformational changes of human hemoglobin (HHb) and lymphocytes by different spectroscopic (intrinsic and synchronous fluorescence spectroscopy and far and near circular dichroism [CD] spectroscopy), docking and cellular (MTT and flow cytometry) investigations. Transmission electron microscopy (TEM) showed that CNP diameter is ~30 nm. The infrared spectrum demonstrated a strong band around 783 cm-1 corresponding to the CNP stretching bond. Fluorescence data revealed that the CNP is able to quench the intrinsic fluorescence of HHb through both dynamic and static quenching mechanisms. The binding constant (Kb ), number of binding sites (n), and thermodynamic parameters over three different temperatures indicated that hydrophobic interactions might play a considerable role in the interaction of CNPs with HHb. Synchronous fluorescence spectroscopy indicated that microenvironmental changes around Trp and Tyr residues remain almost unchanged. CD studies displayed that the regular secondary structure of HHb had no significant changes; however, the quaternary structure of protein is subjected to marginal structural changes. Docking studies showed the larger CNP cluster is more oriented toward experimental data, compared with smaller counterparts. Cellular assays revealed that CNP, at high concentrations (>50 µg/mL), initiated an antiproliferative response through apoptosis induction on lymphocytes. The findings may exhibit that, although CNPs did not significantly perturb the native conformation of HHb, they can stimulate some cellular adverse effects at high concentrations that may limit the medicinal and biological application of CNPs. In other words, CNP application in biological systems should be done at low concentrations. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 24 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 24 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 3 | 13% |
| Researcher | 3 | 13% |
| Student > Master | 3 | 13% |
| Professor | 1 | 4% |
| Unspecified | 1 | 4% |
| Other | 1 | 4% |
| Unknown | 12 | 50% |
| Readers by discipline | Count | As % |
|---|---|---|
| Chemistry | 3 | 13% |
| Biochemistry, Genetics and Molecular Biology | 3 | 13% |
| Agricultural and Biological Sciences | 2 | 8% |
| Environmental Science | 1 | 4% |
| Unspecified | 1 | 4% |
| Other | 2 | 8% |
| Unknown | 12 | 50% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 10 August 2018.
All research outputs
#17,292,294
of 25,385,509 outputs
Outputs from International Journal of Nanomedicine
#2,469
of 4,122 outputs
Outputs of similar age
#220,369
of 341,886 outputs
Outputs of similar age from International Journal of Nanomedicine
#41
of 74 outputs
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