| Title |
In vitro molecular study of wound healing using biosynthesized bacteria nanocellulose/silver nanocomposite assisted by bioinformatics databases
|
|---|---|
| Published in |
International Journal of Nanomedicine, September 2018
|
| DOI | 10.2147/ijn.s164573 |
| Pubmed ID | |
| Authors |
Mona Moniri, Amin Boroumand Moghaddam, Susan Azizi, Raha Abdul Rahim, Saad Wan Zuhainis, Mohammad Navaderi, Rosfarizan Mohamad |
| Abstract |
In recent years, bacterial nanocellulose (BNC) based nanocomposites have been developed to promote healing property and antibacterial activity of BNC wound dressing. Molecular study can help to better understanding about interaction of genes and pathways involved in healing progression. The aim of this study was to prepare bacterial nanocellulose/silver (BNC/Ag) nanocomposite films as ecofriendly wound dressing in order to assess their physical, cytotoxicity and antimicrobial properties. The in vitro molecular study was performed to evaluate expression of genes involved in healing of wounds after treatment with BNC/Ag biofilms. Silver nanoparticles were formed by using Citrullus colocynthis extract within new isolated bacterial nanocellulose (BNC) RM1. The nanocomposites were characterized using X-ray diffraction, Fourier transform infrared, and field emission scanning electron microscopy. Besides, swelling property and Ag release profile of the nanocomposites were studied. The ability of nanocomposites to promote wound healing of human dermal fibroblast cells in vitro was studied. Bioinformatics databases were used to identify genes with important healing effect. Key genes which interfered with healing were studied by quantitative real time PCR. Spherical silver nanoparticles with particle size ranging from 20 to 50 nm were synthesized and impregnated within the structure of BNC. The resulting nanocomposites showed significant antibacterial activities with inhibition zones ranging from 7±0.25 to 16.24±0.09 mm against skin pathogenic bacteria. Moreover, it was compatible with human fibroblast cells (HDF) and could promote in vitro wound healing after 48h. Based on bioinformatics databases, the genes of TGF-β1, MMP2, MMP9, CTNNB1, Wnt4, hsa-miR-29b-3p and hsa-miR-29c-3p played important role in wound healing. The nanocomposites had an effect in expression of the genes in healing. Thus, the BNC/Ag nanocomposite can be used to heal wound in a short period and simple manner. This eco-friendly nanocomposite with excellent antibacterial activities and healing property confirming its utility as potential wound dressings. |
X Demographics
The data shown below were collected from the profiles of 2 X users who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 2 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Scientists | 1 | 50% |
| Members of the public | 1 | 50% |
Mendeley readers
The data shown below were compiled from readership statistics for 90 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 90 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Bachelor | 13 | 14% |
| Student > Master | 12 | 13% |
| Researcher | 9 | 10% |
| Student > Doctoral Student | 7 | 8% |
| Other | 6 | 7% |
| Other | 14 | 16% |
| Unknown | 29 | 32% |
| Readers by discipline | Count | As % |
|---|---|---|
| Materials Science | 9 | 10% |
| Pharmacology, Toxicology and Pharmaceutical Science | 8 | 9% |
| Chemistry | 7 | 8% |
| Agricultural and Biological Sciences | 7 | 8% |
| Biochemistry, Genetics and Molecular Biology | 6 | 7% |
| Other | 15 | 17% |
| Unknown | 38 | 42% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2018.
All research outputs
#20,663,600
of 25,385,509 outputs
Outputs from International Journal of Nanomedicine
#3,128
of 4,122 outputs
Outputs of similar age
#268,908
of 345,739 outputs
Outputs of similar age from International Journal of Nanomedicine
#71
of 88 outputs
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We're also able to compare this research output to 88 others from the same source and published within six weeks on either side of this one. This one is in the 13th percentile – i.e., 13% of its contemporaries scored the same or lower than it.