| Title |
In situ exploration of characteristics of macropinocytosis and size range of internalized substances in cells by 3D-structured illumination microscopy
|
|---|---|
| Published in |
International Journal of Nanomedicine, September 2018
|
| DOI | 10.2147/ijn.s171973 |
| Pubmed ID | |
| Authors |
Jing Jin, Yanting Shen, Biao Zhang, Rong Deng, Dianshuai Huang, Tianqi Lu, Fei Sun, Shuping Xu, Chongyang Liang |
| Abstract |
Macropinocytosis can occur in various types of cells and displays multiple functions. However, real-time observation and characterization of the structures of macropinocytosis on the surface of the cell membrane is not yet possible. Here, we establish a real-time live cell surface imaging method using three-dimensional-structured illumination microscopy. Based on this, observation of the dynamic macropinocytosis process and morphological data of internalized structures on the surface of pancreatic cancer cells were achieved during macropinocytosis. Next, different-sized silica nanoparticles (SiO2 NPs) were used as the scale for identifying the size range of internalized substances of macropinocytosis in pancreatic cancer cells. Our study not only provides a practical method and more structural data for further investigation of macropinocytosis, but also makes deeper understanding of the cell response toward nanomaterials as well as nanodrugs possible. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Members of the public | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 30 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 30 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Student > Ph. D. Student | 8 | 27% |
| Researcher | 4 | 13% |
| Student > Master | 3 | 10% |
| Student > Bachelor | 2 | 7% |
| Unspecified | 1 | 3% |
| Other | 2 | 7% |
| Unknown | 10 | 33% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 6 | 20% |
| Arts and Humanities | 2 | 7% |
| Agricultural and Biological Sciences | 2 | 7% |
| Immunology and Microbiology | 2 | 7% |
| Unspecified | 1 | 3% |
| Other | 5 | 17% |
| Unknown | 12 | 40% |
Attention Score in Context
This research output has an Altmetric Attention Score of 2. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 27 September 2018.
All research outputs
#16,728,456
of 25,385,509 outputs
Outputs from International Journal of Nanomedicine
#2,087
of 4,122 outputs
Outputs of similar age
#211,802
of 345,739 outputs
Outputs of similar age from International Journal of Nanomedicine
#38
of 88 outputs
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