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NMDA receptors are expressed by small-cell lung cancer and are potential targets for effective treatment

Overview of attention for article published in Clinical Pharmacology : Advances and Applications, April 2010
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About this Attention Score

  • In the top 25% of all research outputs scored by Altmetric
  • Among the highest-scoring outputs from this source (#47 of 170)
  • Good Attention Score compared to outputs of the same age (72nd percentile)
  • High Attention Score compared to outputs of the same age and source (80th percentile)

Mentioned by

patent
3 patents

Citations

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45 Dimensions

Readers on

mendeley
41 Mendeley
Title
NMDA receptors are expressed by small-cell lung cancer and are potential targets for effective treatment
Published in
Clinical Pharmacology : Advances and Applications, April 2010
DOI 10.2147/cpaa.s6262
Pubmed ID
Authors

William William G. North

Abstract

We previously showed that functional N-methyl-D-aspartate (NMDA) receptors are expressed by human neuroblastoma cells. In this study we demonstrate functional NMDAR1 and NMDAR2 receptors are expressed by small-cell lung cancer (SCLC) classical cell lines NCI H146, NCI H345, and DMS 53, by variant cell line NCI H82, and by most SCLC tumors, and that these receptors are important for the growth of human SCLC tumor xenografts in mice. Reverse transcription-polymerase chain reaction demonstrated mRNA for both receptors, with sequences identical to those for human mRNAs, are expressed in all four cell lines, and these generated proteins of the expected sizes 120 and 170 kDa. Cell viability tests showed cell growth was significantly (P < 0.0001) impaired by NMDAR1 antagonists MK-801 and memantine. Ifenprodil and Ro25-6981, NMDAR2B antagonists at the polyamine site, also significantly (P < 0.001) inhibited the growth/survival of these cells. Alternatively, the glycine-binding antagonist, L701, 324, increased viability to 140% and 120% in NCI H345 and NCI H82 cells after 48 hours of incubation. Immunohistochemistry of SCLC tumors with our polyclonal antibodies gave specific positive staining for the NMDAR1 receptor in 8 of 10 tissues examined. Small amounts of these same antibodies significantly reduced the growth of NCI-H345 cells up to 25% (P < 0.001). When NCI H345 cells were grown as tumor xenografts in mice, the growth of these tumors was reduced by 60% (P < 0.001) by treatments with MK-801 over five days. All of these data point to active NMDAR receptors possibly having an important influence on SCLC growth and survival.

Mendeley readers

The data shown below were compiled from readership statistics for 41 Mendeley readers of this research output. Click here to see the associated Mendeley record.

Geographical breakdown

Country Count As %
Germany 1 2%
Unknown 40 98%

Demographic breakdown

Readers by professional status Count As %
Student > Ph. D. Student 8 20%
Professor > Associate Professor 5 12%
Professor 5 12%
Other 4 10%
Researcher 4 10%
Other 11 27%
Unknown 4 10%
Readers by discipline Count As %
Biochemistry, Genetics and Molecular Biology 11 27%
Agricultural and Biological Sciences 11 27%
Medicine and Dentistry 8 20%
Neuroscience 5 12%
Chemistry 1 2%
Other 0 0%
Unknown 5 12%

Attention Score in Context

This research output has an Altmetric Attention Score of 6. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 22 September 2022.
All research outputs
#4,682,780
of 22,731,677 outputs
Outputs from Clinical Pharmacology : Advances and Applications
#47
of 170 outputs
Outputs of similar age
#20,282
of 95,522 outputs
Outputs of similar age from Clinical Pharmacology : Advances and Applications
#1
of 5 outputs
Altmetric has tracked 22,731,677 research outputs across all sources so far. Compared to these this one has done well and is in the 76th percentile: it's in the top 25% of all research outputs ever tracked by Altmetric.
So far Altmetric has tracked 170 research outputs from this source. They receive a mean Attention Score of 4.9. This one has gotten more attention than average, scoring higher than 70% of its peers.
Older research outputs will score higher simply because they've had more time to accumulate mentions. To account for age we can compare this Altmetric Attention Score to the 95,522 tracked outputs that were published within six weeks on either side of this one in any source. This one has gotten more attention than average, scoring higher than 72% of its contemporaries.
We're also able to compare this research output to 5 others from the same source and published within six weeks on either side of this one. This one has scored higher than all of them