| Title |
In vitro modeling of COPD inflammation and limitation of p38 inhibitor — SB203580
|
|---|---|
| Published in |
International Journal of Chronic Obstructive Pulmonary Disease, April 2016
|
| DOI | 10.2147/copd.s99810 |
| Pubmed ID | |
| Authors |
Aihong Meng, Xiaopeng Zhang, Siyu Wu, Mingxia Wu, Jing Li, Xixin Yan, Kamilla Kopec-Harding, Jiakai Wu |
| Abstract |
Systemic inflammation and steroid resistance are the hallmarks of COPD. We examined the impact of p38 inhibitor (SB203580) in in vitro assays of systemic inflammation using pulmonary cells and patients' sera. Data from 66 COPD patients and 15 age-/sex-matched healthy controls were compared. Interleukin-10 (IL-10), tumor necrosis factor-α (TNF-α), and CCL5 were measured in serum samples and culture media from peripheral blood mononuclear cells. The impact of sera on IL-10 and CCL5 expression in alveolar macrophage cell line (MH-S) was examined. The in vitro effects of SB203580 on lipopolysaccharide-induced inflammation were investigated. Peripheral blood mononuclear cells from Global initiative for chronic Obstructive Lung Disease (GOLD) D patients produced more CCL5 and TNF-α, and less IL-10 compared to GOLD A-C patients. SB203580 treatment suppressed CCL5 and TNF-α and stimulated IL-10 production; however, the effect of SB203580 on IL-10 was lower in the COPD group. Culture of MH-S cells with COPD serum showed a significant increase in CCL5 and a significant decrease in IL-10 compared to healthy serum. This effect was not suppressed with SB203580 treatment. COPD serum has a potent proinflammatory effect on pulmonary cells. Inhibition of p38 phoshorylation had a limited effect in restoring impaired lymphocyte function and suppressing inflammation induced by COPD serum, implying important p38-independent inflammatory mechanisms in COPD. |
X Demographics
The data shown below were collected from the profile of 1 X user who shared this research output. Click here to find out more about how the information was compiled.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 1 | 100% |
Demographic breakdown
| Type | Count | As % |
|---|---|---|
| Practitioners (doctors, other healthcare professionals) | 1 | 100% |
Mendeley readers
The data shown below were compiled from readership statistics for 33 Mendeley readers of this research output. Click here to see the associated Mendeley record.
Geographical breakdown
| Country | Count | As % |
|---|---|---|
| Unknown | 33 | 100% |
Demographic breakdown
| Readers by professional status | Count | As % |
|---|---|---|
| Researcher | 6 | 18% |
| Student > Master | 4 | 12% |
| Student > Ph. D. Student | 4 | 12% |
| Other | 3 | 9% |
| Student > Bachelor | 2 | 6% |
| Other | 4 | 12% |
| Unknown | 10 | 30% |
| Readers by discipline | Count | As % |
|---|---|---|
| Biochemistry, Genetics and Molecular Biology | 8 | 24% |
| Pharmacology, Toxicology and Pharmaceutical Science | 4 | 12% |
| Agricultural and Biological Sciences | 4 | 12% |
| Nursing and Health Professions | 2 | 6% |
| Engineering | 2 | 6% |
| Other | 1 | 3% |
| Unknown | 12 | 36% |
Attention Score in Context
This research output has an Altmetric Attention Score of 1. This is our high-level measure of the quality and quantity of online attention that it has received. This Attention Score, as well as the ranking and number of research outputs shown below, was calculated when the research output was last mentioned on 02 May 2016.
All research outputs
#20,656,161
of 25,373,627 outputs
Outputs from International Journal of Chronic Obstructive Pulmonary Disease
#2,078
of 2,577 outputs
Outputs of similar age
#234,500
of 314,719 outputs
Outputs of similar age from International Journal of Chronic Obstructive Pulmonary Disease
#59
of 68 outputs
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