Title |
In vitro and in vivo targeting imaging of pancreatic cancer using a Fe3O4@SiO2 nanoprobe modified with anti-mesothelin antibody
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Published in |
International Journal of Nanomedicine, May 2016
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DOI | 10.2147/ijn.s104501 |
Pubmed ID | |
Authors |
Fang Liu, Wenjun Le, Tianxiao Mei, Tiegong Wang, Luguang Chen, Yi Lei, Shaobin Cui, Bingdi Chen, Zheng Cui, Chengwei Shao |
Abstract |
Pancreatic cancer is a highly malignant disease with a 5-year survival rate <5% mainly due to lack of early diagnosis and effective therapy. In an effort to improve the early diagnostic rate of pancreatic cancer, a nanoprobe Fe3O4@SiO2 modified with anti-mesothelin antibody (A-MFS) was prepared to target cells and tumor tissues highly expressing mesothelin in vitro (human pancreatic cancer cell line SW1990) and in vivo (subcutaneously transplanted tumors) studies. The A-MFS probe was successfully prepared and was spherical and uniform with a hydrodynamic diameter between 110 and 130 nm. Cell Counting Kit-8 testing indicated that A-MFS was nontoxic in vitro and in vivo studies. The in vitro study showed that the A-MFS probe specifically targeted SW1990 cells with high mesothelin expression. The in vivo study was conducted in Siemens 3.0 T magnetic resonance imaging. The average T2-weighted signal values of the xenografts were 966.533±31.56 before injecting A-MFS and 691.133±56.84 before injecting saline solution. After injection of 0.1 mL A-MFS via nude mouse caudal vein for 2.5 hours, the average T2-weighted signal of the xenograft decreased by 342.533±42.6. The signal value decreased by -61.233±33.9 and -58.7±19.4 after injection of the saline and Fe3O4@SiO2. The decrease of tumor signal by A-MFS was much more significant than that by saline and Fe3O4@SiO2 (P<0.05). The results demonstrated the high stability and nontoxicity of A-MFS, which effectively targeted pancreatic cancer in vitro and in vivo. A-MFS is a promising agent for diagnosis of pancreatic cancer. |
X Demographics
Geographical breakdown
Country | Count | As % |
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Canada | 1 | 17% |
United Kingdom | 1 | 17% |
Unknown | 4 | 67% |
Demographic breakdown
Type | Count | As % |
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Members of the public | 4 | 67% |
Practitioners (doctors, other healthcare professionals) | 2 | 33% |
Mendeley readers
Geographical breakdown
Country | Count | As % |
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Unknown | 27 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Student > Ph. D. Student | 5 | 19% |
Student > Bachelor | 4 | 15% |
Student > Doctoral Student | 3 | 11% |
Researcher | 3 | 11% |
Student > Master | 2 | 7% |
Other | 1 | 4% |
Unknown | 9 | 33% |
Readers by discipline | Count | As % |
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Pharmacology, Toxicology and Pharmaceutical Science | 3 | 11% |
Chemical Engineering | 2 | 7% |
Medicine and Dentistry | 2 | 7% |
Biochemistry, Genetics and Molecular Biology | 2 | 7% |
Agricultural and Biological Sciences | 1 | 4% |
Other | 6 | 22% |
Unknown | 11 | 41% |