Title |
Ultrasonic Nanoemulsification of Cuminum cyminum Essential Oil and Its Applications in Medicine
|
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Published in |
International Journal of Nanomedicine, February 2020
|
DOI | 10.2147/ijn.s230893 |
Pubmed ID | |
Authors |
M Joyce Nirmala, Latha Durai, Kagitala Anvesh Rao, R Nagarajan |
Abstract |
Cumin seed oil (extracted from Cuminum cyminum) has many applications but conclusive evidence of its therapeutic uses has not been presented. This study has explored the anticancer and antibacterial properties of the seed oil. The cumin nanoemulsion was prepared with Tween 80 non-ionic surfactant employing ultra-sonication technology. The anticancer activity of the nanoscale-based emulsion was evaluated through cell viability (MTT), antiproliferation evaluation through clonogenic assay, and apoptosis through Annexin V-FITC assay. Agar well diffusion was used to study the antimicrobial activity, and this was supported by membrane integrity analysis. A thorough study of process parameters, aimed at obtaining the optimal surface concentration and emulsification time, was completed. GC-MS data indicated cumaldehyde as a major component. The resultant droplet diameter after a sonication time of 5 min was 10.4 ± 0.5 nm. MTT assay revealed the IC50 value at 1.5 µL/mL and the early induction of apoptosis was evident. Tongue carcinoma cell line treated with cumin nanoemulsion presented a diminished colony formation. The nanoemulsion exhibited significant antibacterial activity against S. aureus. A significant cytoplasmic leakage was observed on treatment with cumin nanoemulsion. The consequences of the analysis projected cumin as a potential component for cancer therapy. This study provides definitive evidence for cumin essential oil nanoemulsion as a legitimate plant-based medicine that can bypass the drawbacks of the present aggressive treatment of cancer, can overcome the antimicrobial resistance, and can also meet all prerequisites. |
Mendeley readers
Geographical breakdown
Country | Count | As % |
---|---|---|
Unknown | 92 | 100% |
Demographic breakdown
Readers by professional status | Count | As % |
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Researcher | 10 | 11% |
Student > Master | 8 | 9% |
Student > Postgraduate | 7 | 8% |
Student > Bachelor | 6 | 7% |
Student > Ph. D. Student | 6 | 7% |
Other | 12 | 13% |
Unknown | 43 | 47% |
Readers by discipline | Count | As % |
---|---|---|
Agricultural and Biological Sciences | 9 | 10% |
Pharmacology, Toxicology and Pharmaceutical Science | 8 | 9% |
Medicine and Dentistry | 6 | 7% |
Chemistry | 6 | 7% |
Veterinary Science and Veterinary Medicine | 4 | 4% |
Other | 11 | 12% |
Unknown | 48 | 52% |